IL-4 antibody | B03-6A7

100% Secure


Mouse anti Human Interleukin-4:Biotin

Product Type
Monoclonal Antibody
Clone
B03-6A7
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6241B
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human interleukin-4 antibody, clone B03-6A7, recognizes interleukin-4 (IL-4) also known as B-cell stimulatory factor 1, binetrakin and lymphocyte stimulatory factor 1. IL-4 is a cytokine largely expressed and secreted by CD4+ T helper cells, basophils, eosinophils, mast cells and natural killer cells (Junttila 2018). IL-4 is expressed at low levels under physiological conditions, with the levels influenced by hormones, cellular/oxidative stress, infection and inflammation (McCormick & Heller 2015).

IL-4 plays important roles in both physiological and pathological conditions such as Th2 cell-mediated immunity, IgE and IgG1 class switching in B cells and tissue repair and homeostasis through alternative macrophage activation (Gadani et al. 2013). The effect of IL-4 is mediated through the receptor IL-4Rα. Upon binding of IL-4 to IL-4Rα, the complex binds to a secondary receptor chain, IL-2Rγc or IL-13Rα1. This receptor complex is then able to induce intracellular signaling via JAK1 and JAK3 (Junttila 2018).

The biotinylated Mouse anti Human interleukin-4 antibody, clone B03-6A7 (MCA6241B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human interleukin-4 antibody, clone B04-9E1 (MCA6240GA) as the capture antibody.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
E. coli derived recombinant human full length interleukin-4 (His25-Ser153)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
P05112
Entrez Gene
IL4
GO Terms
GO:0005125 cytokine activity
GO:0005136 interleukin-4 receptor binding
GO:0005615 extracellular space
GO:0006935 chemotaxis
GO:0006968 cellular defense response
GO:0008083 growth factor activity
GO:0008203 cholesterol metabolic process
GO:0016563 transcription activator activity
GO:0016564 transcription repressor activity
GO:0030183 B cell differentiation
GO:0030890 positive regulation of B cell proliferation
GO:0042092 type 2 immune response
GO:0043066 negative regulation of apoptosis
GO:0045189 connective tissue growth factor biosynthetic process
GO:0045348 positive regulation of MHC class II biosynthetic process
GO:0045671 negative regulation of osteoclast differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048295 positive regulation of isotype switching to IgE isotypes
GO:0048304 positive regulation of isotype switching to IgG isotypes
Regulatory
For research purposes only

Applications of IL-4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human Interleukin-4 MCA6240GA E 0.1 mg loader

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra