Mouse anti Human interleukin-34 antibody, clone C03-5C3, recognizes interleukin-34 (IL-34), also known as C16orf77. IL-34 is an inflammatory cytokine with the ability to induce macrophage activation and migration (Zhou et al. 2016). This protein is produced by multiple cell types including endothelial cells, adipocytes, neurons, macrophages, fibroblasts, and epithelial cells (Franzè et al. 2020). IL-34 is involved with development and maintenance of multiple macrophage subsets, including osteoclasts, Langerhans cells, and microglia. If macrophages are cultured in the presence of IL-34, these cells show immunosuppressive properties (Lindau et al. 2018). Due to its inflammatory role, IL-34 dysregulation can be involved with inflammatory bowel disease, rheumatoid arthritis, heart failure, and kidney diseases (Cheng et al. 2017). IL-34 is overexpressed in several cancer types, and tumor cells can produce and respond to this cytokine (Franzè et al. 2020)

The biotinylated Mouse anti Human interleukin-34 antibody, clone C03-5C3 (MCA6215B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human interleukin-34 antibody, clone D02-3A7 (MCA6214GA) as the capture antibody.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1.0 ug/ml