IL-34 antibody | C03-5C3

ISO 9001 Quality Assurance Manufacturing | Print

Anti Human INTERLEUKIN-34 Antibody, clone C03-5C3 thumbnail image 1

Anti Human INTERLEUKIN-34 Antibody, clone C03-5C3 gallery image 1 — Sandwich ELISA analysis of human IL-34 using Mouse anti Human interleukin-34 (MCA6214GA) as a capture reagent and biotinylated Mouse anti Human interleukin-34 (**MCA6215B**) as a detection reagent with recombinant human IL-34 as the antigen. Detection was performed using HRP conjugated streptavidin and substrate.

Filter by Application:

Mouse anti Human INTERLEUKIN-34:Biotin

Product Type: Monoclonal Antibody
Clone: C03-5C3
Isotype: IgG2b
Specificity: IL-34

Quick Links:

Applications
Useful Reagents
Storage Information
Batch/Lot Specific Datasheets

Antibody Quality and Performance Guarantee

Unrivaled Technical Support

Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
MCA6215B Datasheet Datasheet SDS SDS	E	0.1 mg		Log in	Get Quote
	Log in

Mouse anti Human interleukin-34 antibody, clone C03-5C3, recognizes interleukin-34 (IL-34), also known as C16orf77. IL-34 is an inflammatory cytokine with the ability to induce macrophage activation and migration (Zhou et al. 2016). This protein is produced by multiple cell types including endothelial cells, adipocytes, neurons, macrophages, fibroblasts, and epithelial cells (Franzè et al. 2020). IL-34 is involved with development and maintenance of multiple macrophage subsets, including osteoclasts, Langerhans cells, and microglia. If macrophages are cultured in the presence of IL-34, these cells show immunosuppressive properties (Lindau et al. 2018). Due to its inflammatory role, IL-34 dysregulation can be involved with inflammatory bowel disease, rheumatoid arthritis, heart failure, and kidney diseases (Cheng et al. 2017). IL-34 is overexpressed in several cancer types, and tumor cells can produce and respond to this cytokine (Franzè et al. 2020)

The biotinylated Mouse anti Human interleukin-34 antibody, clone C03-5C3 (MCA6215B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human interleukin-34 antibody, clone D02-3A7 (MCA6214GA) as the capture antibody.

Target Species: Human
Product Form: Purified IgG conjugated to Biotin - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Immunogen: E. coli derived recombinant human full length interleukin-34 (Asn21-Pro242)
Approx. Protein Concentrations: IgG concentration 0.1 mg/ml
Fusion Partners: Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1.0 ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description	Product Code	Applications	Pack Size	Your Price
Mouse anti Human INTERLEUKIN-34	MCA6214GA	E	0.1 mg	Log in	Get Quote
List Price	Your Price
	Log in
Description	Mouse anti Human INTERLEUKIN-34

UniProt: Q6ZMJ4
Entrez Gene: IL34
GO Terms: GO:0001934 positive regulation of protein phosphorylation; GO:0005125 cytokine activity; GO:0005157 macrophage colony-stimulating factor receptor binding; GO:0005615 extracellular space; GO:0008083 growth factor activity; GO:0008284 positive regulation of cell proliferation