IL-34 antibody | C03-5C3

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Mouse anti Human INTERLEUKIN-34:Biotin

Product Type
Monoclonal Antibody
Clone
C03-5C3
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA6215B
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human interleukin-34 antibody, clone C03-5C3, recognizes interleukin-34 (IL-34), also known as C16orf77. IL-34 is an inflammatory cytokine with the ability to induce macrophage activation and migration (Zhou et al. 2016). This protein is produced by multiple cell types including endothelial cells, adipocytes, neurons, macrophages, fibroblasts, and epithelial cells (Franzè et al. 2020). IL-34 is involved with development and maintenance of multiple macrophage subsets, including osteoclasts, Langerhans cells, and microglia. If macrophages are cultured in the presence of IL-34, these cells show immunosuppressive properties (Lindau et al. 2018). Due to its inflammatory role, IL-34 dysregulation can be involved with inflammatory bowel disease, rheumatoid arthritis, heart failure, and kidney diseases (Cheng et al. 2017). IL-34 is overexpressed in several cancer types, and tumor cells can produce and respond to this cytokine (Franzè et al. 2020)

The biotinylated Mouse anti Human interleukin-34 antibody, clone C03-5C3 (MCA6215B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human interleukin-34 antibody, clone D02-3A7 (MCA6214GA) as the capture antibody.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
E. coli derived recombinant human full length interleukin-34 (Asn21-Pro242)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
Q6ZMJ4
Entrez Gene
IL34
GO Terms
GO:0001934 positive regulation of protein phosphorylation
GO:0005125 cytokine activity
GO:0005157 macrophage colony-stimulating factor receptor binding
GO:0005615 extracellular space
GO:0008083 growth factor activity
GO:0008284 positive regulation of cell proliferation
Regulatory
For research purposes only

Applications of IL-34 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human INTERLEUKIN-34 MCA6214GA E 0.1 mg loader

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