Mouse anti Human interleukin-34 antibody, clone C03-5C3, recognizes interleukin-34 (IL-34), also known as C16orf77. IL-34 is an inflammatory cytokine with the ability to induce macrophage activation and migration (
Zhou et al. 2016). This protein is produced by multiple cell types including endothelial cells, adipocytes, neurons, macrophages, fibroblasts, and epithelial cells (
Franzè et al. 2020). IL-34 is involved with development and maintenance of multiple macrophage subsets, including osteoclasts, Langerhans cells, and microglia. If macrophages are cultured in the presence of IL-34, these cells show immunosuppressive properties (
Lindau et al. 2018). Due to its inflammatory role, IL-34 dysregulation can be involved with inflammatory bowel disease, rheumatoid arthritis, heart failure, and kidney diseases (
Cheng et al. 2017). IL-34 is overexpressed in several cancer types, and tumor cells can produce and respond to this cytokine (
Franzè et al. 2020)
The biotinylated Mouse anti Human interleukin-34 antibody, clone C03-5C3 (
MCA6215B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human interleukin-34 antibody, clone D02-3A7 (
MCA6214GA) as the capture antibody.
Applications of IL-34 antibody
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name |
Verified |
Min Dilution |
Max Dilution |
ELISA |
|
1.0 ug/ml |
|
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.