IL-31 antibody | A12-2F7

Mouse anti Human Interleukin-31:Biotin

Product Type
Monoclonal Antibody
Clone
A12-2F7
Isotype
IgG1
Specificity
IL-31

Product Code Applications Pack Size List Price Your Price Qty
MCA6247B
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
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Mouse anti Human interleukin-31 antibody, clone A12-2F7, recognizes interleukin-31 (IL-31), a member of the gp130/IL-6 family of proteins. It is a cytokine that has pro-inflammatory properties. IL-31 is mainly produced by CD4+ T helper and mast cells (Di Salvo et al. 2018). IL-31 acts upon both immune and non-immune cells, regulating signaling of pro-inflammatory cytokines, cell proliferation and tissue remodeling. The effect of IL-31 is mediated by its receptor IL-31RA. Upon binding to this receptor, the JAK/STAT, PI3K/AKT and MAPK pathways can be activated (Zhang et al. 2008).

IL-31 has been implicated in chronic inflammation, with elevated levels detected in various skin diseases, Crohn’s disease and ulcerative colitis. Higher IL-31 levels in serum have also been positively correlated with asthma severity (Lai et al. 2016).

The biotinylated Mouse anti Human interleukin-31 antibody, clone A12-2F7 (MCA6247B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human interleukin-31 antibody, clone A01-1E4/A09.2-2E6 (MCA6246GA) as the capture antibody.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
E. coli derived recombinant human full length interleukin-31 (Ser24-Thr164)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Human Interleukin-31 MCA6246GA E 0.1 mg loader
List Price Your Price
loader
Description Mouse anti Human Interleukin-31

UniProt
Q6EBC2
Entrez Gene
IL31
GO Terms
GO:0005125 cytokine activity
GO:0005615 extracellular space
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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