Cytochrome P450 Aromatase antibody | H4

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Mouse anti Human Cytochrome P450 Aromatase

Product Type
Monoclonal Antibody
Clone
H4
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2077S IF P WB 1 ml
Mouse anti Human Cytochrome P450 aromatase antibody, clone H4 recognizes a conserved epitope within cytochrome P450 aromatase (P450 arom). P450 arom plays an important role in estrogen biosynthesis and is highly conserved amongst mammals.
P450 arom is highly expressed in placental tissue. For tissues where there may be low expression of P450 arom, the use of microsomal extracts may improve the staining for Western blots using Mouse anti Human Cytochrome P450 aromatase antibody, clone H4 (Turner et al. 2002).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Marmoset
Chicken
Mouse
Pig
Baboon
Bovine
Horse
Great fruit eating bat
Rabbit
Sheep
Collared peccary
Goat
Giraffe
Minke whale
Bryde's whale
Sei whale
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Concentrated Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
Synthetic peptide corresponding to amino acids 376 - 390 of human aromatase.
Fusion Partners
Spleen cells from immunised Balb/c were fused with with cells of the mouse SP20 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P11511 Related reagents
Entrez Gene
CYP19A1 Related reagents
GO Terms
GO:0009055 electron carrier activity
GO:0005624 membrane fraction
GO:0005789 endoplasmic reticulum membrane
GO:0055114 oxidation-reduction process
GO:0006703 estrogen biosynthetic process
GO:0006805 xenobiotic metabolic process
GO:0008395 steroid hydroxylase activity
GO:0019825 oxygen binding
GO:0020037 heme binding
GO:0070330 aromatase activity
Regulatory
For research purposes only

Applications of Cytochrome P450 Aromatase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Paraffin
Western Blotting 1/250
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human placenta
Western Blotting
Mouse anti Human p450 Aromatase antibody, clone H4 detects a band of approximately 55 kDa in human placental extracts.

Application Based External Images

Flow Cytometry

Immunofluorescence

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for Cytochrome P450 Aromatase antibody

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    Mol Endocrinol. 21 (3): 651-63.
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  7. Wu, Y.G. et al. (2011) Testosterone, not 5{alpha}-Dihydrotestosterone, Stimulates LRH-1 Leading to FSH-Independent Expression of Cyp19 and P450scc in Granulosa Cells.
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  8. Lu, Y. et al. (2011) BRCA1/BARD1 complex interacts with steroidogenic factor 1--A potential mechanism for regulation of aromatase expression by BRCA1.
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