CXCL9 antibody | C09-9H7

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Mouse anti Human CXCL9:Biotin

Product Type
Monoclonal Antibody
Clone
C09-9H7
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6253B
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human CXCL9 antibody, clone C09-9H7, recognizes, C-X-C motif chemokine 9 (CXCL9), also known as Monokine induced by Interferon-Gamma (MIG) and small inducible cytokine B9.

Synthesis of CXCL9 is induced by IFN-gamma and it mediates lymphoytic infiltration to focal sites and inhibits tumor growth (Tokunaga et al. 2018). CXCL9 is not detectable under physiological conditions in non-lymphoid tissues. However in response to inflammation or injury, IFN-gamma induces CXCL9 production in blood and tissue cells. CXCL9 is a ligand of CXCR3, a classic 7 transmembrane G protein coupled receptor. Upon binding to this receptor the signaling pathways Src, PI3k and MAPK can be activated (Koper et al. 2018).

The biotinylated Mouse anti Human CXCL9 antibody, clone C09-9H7 (MCA6253B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human CXCL9 antibody, clone C04-3E4 (MCA6252GA) as the capture antibody.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
E. coli derived recombinant human full length CXCL9 (Thr23-Thr125)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
Q07325
Entrez Gene
CXCL9
GO Terms
GO:0005615 extracellular space
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0006968 cellular defense response
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0007267 cell-cell signaling
GO:0008009 chemokine activity
Regulatory
For research purposes only

Applications of CXCL9 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human CXCL9 MCA6252GA E 0.1 mg loader

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