CXCL16 antibody | C19-5F9

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Mouse anti Human CXCL16:Biotin

Product Type
Monoclonal Antibody
Clone
C19-5F9
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6223B
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human CXCL16 antibody, clone C19-5F9, recognizes C-X-C motif Chemokine 16 (CXCL16). Unlike most chemokines, CXCL16 is a membrane bound ligand anchored through transmembrane domains. Although a soluble form of CXCL16 is generated through proteolytic cleavage. Transmembrane CXCL16 is mainly expressed on macrophages, dendritic cells, B cells and various tumor cells. In this form, it acts as an adhesion molecule though the binding of its receptor CXCR6 expressed on other cells including T cells. Transmembrane CXCL16 also binds gram positive and gram negative bacteria, playing a role in innate and adaptive immune responses (Shimaoka et al. 2003).

Soluble CXCL16 has chemoattractant activity, and elicits this response through the receptor CXCR6.There is also mounting evidence suggesting an additional role in tumor progression. With a number of studies suggesting CXCL16 as a biomarker for the evaluation of some tumor type prognoses (Richardsen et al. 2015, Shibata et al. 2020, Gooden et al. 2014).

The biotinylated Mouse anti Human CXCL16 antibody, clone C19-5F9 (MCA6223B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human CXCL16 antibody, clone B06-3H6 (MCA6222GA) as the capture antibody.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
E. coli derived recombinant human full length CXCL16 (Asn49-Pro137)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9H2A7
Entrez Gene
CXCL16
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005041 low-density lipoprotein receptor activity
GO:0005044 scavenger receptor activity
GO:0005615 extracellular space
GO:0006898 receptor-mediated endocytosis
GO:0008009 chemokine activity
GO:0030307 positive regulation of cell growth
GO:0030335 positive regulation of cell migration
GO:0034341 response to interferon-gamma
GO:0034612 response to tumor necrosis factor
GO:0048247 lymphocyte chemotaxis
Regulatory
For research purposes only

Applications of CXCL16 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human CXCL16 MCA6222GA E 0.1 mg loader

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