Complement Factor H antibody | C01-4A10
Mouse anti Human Complement Factor H:Biotin
- Product Type
- Monoclonal Antibody
- Clone
- C01-4A10
- Isotype
- IgG1
- Specificity
- Complement Factor H
| Mouse anti Human complement Factor H antibody, clone C01-4A10, recognizes, complement factor H, also known as H Factor1. Complement factor H is a major regulatory protein of the alternative activation pathway of the complement cascade. It regulates this pathway by targeting C3 convertase (C3bBb) for degradation and by acting as a co-factor in the cleavage and inactivation of C3b. Complement factor H is a soluble ~155 kDa glycoprotein present in serum at a relatively high concentration, averaging between 200 – 800 ug/ml. It is also expressed locally by many cells types including epithelial cells and platelets (Ferreira et al. 2010, Parente et al. 2017). Due to the major regulatory functionality of complement factor H, mutations and polymorphisms of the encoding gene are associated with inappropriate recognition of “self” cell markers in patients and consequently results in complement mediated tissue damage and disease. These mutations are associated with various diseases including atypical haemolytic uremic syndrome and C3 glomerulophathy (Martinez-Barricate et al. 2008, Cserhalmi et al. 2017). The biotinylated Mouse anti Human complement factor H antibody, clone C01-4A10 (MCA6229B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human complement factor H antibody, clone C06-2F3 (MCA6228GA) as the capture antibody. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Purified native human complement factor H
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
- ELISA Matched Pair(s)
-
ELISA Matched Pair-Capture Antibody:
MCA6228GA
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1.0 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
MCA6229B
100005425
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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