Chimeric Human IgE anti NP antibody | JW8/1
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|The immunoglobulin heavy chain has been produced by the linking of the antigen-binding, variable region genes of a mouse hybridoma to human constant region genes by in vitro DNA recombination procedures. The resulting chimeric antibody is subsequently expressed by the myeloma cell-line J558L after transfection. (The J558L cell-line self secretes a lambda light chain but no heavy chain). Thus a chimeric human IgE antibody specific for NP has been produced.|
- Target Species
- Product Form
- Tissue Culture Supernatant - liquid
- Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
- Buffer Solution
- None present
- Preservative Stabilisers
0.09% Sodium Azide
- Hapten, 4-hydroxy-3-nitrophenylacetyl (NP).
- Fusion Partners
- Plasmids containing chimaeric heavy chain gene were fused with cells of the J558L mouse myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Functional Assays 1|
- 1 This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
References for Chimeric Human IgE anti NP antibody
Neuberger, M.S. et al. (1984) Recombinant antibodies possessing novel effector functions.
Nature. 312 (5995): 604-8.
Sayers, I. et al. (2004) The importance of Lys-352 of human immunoglobulin E in FcepsilonRII/CD23 recognition.
J Biol Chem. 279: 35320-5.
Neuberger, M.S. et al. (1985) A hapten-specific chimaeric IgE antibody with human physiological effector function.
Nature. 314 (6008): 268-70.
Sallmann, E. et al. (2011) High-Affinity IgE Receptors on Dendritic Cells Exacerbate Th2-Dependent Inflammation.
J Immunol. 187: 164-71.
Xu, D. et al. (2012) RN486, a selective Bruton's tyrosine kinase inhibitor, abrogates immune hypersensitivity responses and arthritis in rodents.
J Pharmacol Exp Ther. 341 (1): 90-103.
Vangelista, L. et al. (2005) Membrane IgE binds and activates Fc epsilon RI in an antigen-independent manner.
J Immunol. 174: 5602-11.
Kulka, M. and Metcalfe, D.D. (2004) High-resolution tracking of cell division demonstrates differential effects of TH1 and TH2 cytokines on SCF-dependent human mast cell production in vitro: correlation with apoptosis and Kit expression.
Blood. 105: 592-9.
Novak, N. et al. (2003) Evidence for a differential expression of the FcepsilonRIgamma chain in dendritic cells of atopic and nonatopic donors.
J Clin Invest. 111: 1047-56.
View The Latest Product References
Sawada, J. et al. (2005) Stem cell factor has a suppressive activity to IgE-mediated chemotaxis of mast cells.
J Immunol. 174: 3626-32.
Ferguson, G.D. et al. (2016) A Novel Triazolopyridine-Based Spleen Tyrosine Kinase Inhibitor That Arrests Joint Inflammation.
PLoS One. 11 (1): e0145705.
Shirley D et al. (2016) Resveratrol preferentially inhibits IgE-dependent PGD2 biosynthesis but enhances TNF production from human skin mast cells.
Biochim Biophys Acta. pii: S0304-4165(16)00015-5.
Burton Oliver T. et al. (2016) A humanized mouse model of anaphylactic peanut allergy
Journal of Allergy and Clinical Immunology. Jun 08 [Epub ahead of print]
Troupin, A. et al. (2016) A Role for Human Skin Mast Cells in Dengue Virus Infection and Systemic Spread.
J Immunol. 197 (11): 4382-4391.
Bratke, K. et al. (2017) Differential regulation of PD-1 and its ligands in allergic asthma.
Clin Exp Allergy. 47 (11): 1417-25.
McHale, C. et al. (2018) Interleukin-6 potentiates FcεRI-induced PGD 2 biosynthesis and induces VEGF from human in situ -matured skin mast cells
Biochimica et Biophysica Acta (BBA) - General Subjects. 1862 (5): 1069-78.
Shan, M. et al. (2018) Secreted IgD Amplifies Humoral T Helper 2 Cell Responses by Binding Basophils via Galectin-9 and CD44.
Immunity. 49 (4): 709-724.e8.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"View all Anti-Human Products
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