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Chimeric Human IgE anti NP antibody | JW8/1

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Anti NP Antibody, clone JW8/1 thumbnail image 1
Anti NP Antibody, clone JW8/1 gallery image 1
Published customer image:
Chimeric Human IgE anti NP antibody clone JW8/1 (MCA333S) used for stimulation of LAD2 cells-in vitro followed by evaluation of β-hexosaminidase activity.
Image caption:
FcR-dependent functional activity in primary human immune cells is inhibited by CC-509. When stimulated through the indicated FcR, CC-509 inhibits functional activity in (A) primary human macrophage, (B) primary human B-cells and (C) the LAD2 human mast cell line. (D) Left panel: CC-509 inhibits CD63 upregulation on basophils stimulated through indicated FcR in human whole blood. Right panel: Representative flow-cytometry plot showing the number of events and CD63 mean fluorescence intensity (MFI) on unstimulated (brown trace) or stimulated basophils in the presence of 0.1 μM (blue trace), 1.0 μM (green trace), or 10.0 μM (red trace) CC-509. IC50 values (μM) are indicated in upper-right portion of each panel. All data expressed as percent of DMSO-treated control, mean ± SD.

From: Ferguson GD, Delgado M, Plantevin-Krenitsky V, Jensen-Pergakes K, Bates RJ, Torres S, et al. (2016)
A Novel Triazolopyridine-Based Spleen Tyrosine Kinase Inhibitor That Arrests Joint Inflammation.
PLoS ONE 11(1): e0145705.

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Chimeric Human IgE anti NP

Product Type
Monoclonal Antibody
Clone
JW8/1
Isotype
IgE
Specificity
Chimeric Human IgE anti NP

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Product Code Applications Pack Size List Price Your Price Qty
MCA333S
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The immunoglobulin heavy chain has been produced by the linking of the antigen-binding, variable region genes of a mouse hybridoma to human constant region genes by in vitro DNA recombination procedures. The resulting chimeric antibody is subsequently expressed by the myeloma cell-line J558L after transfection. (The J558L cell-line self secretes a lambda light chain but no heavy chain). Thus a chimeric human IgE antibody specific for NP has been produced.

Target Species
Human
Product Form
Tissue Culture Supernatant - liquid
Preparation
Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
Buffer Solution
None present
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Hapten, 4-hydroxy-3-nitrophenylacetyl (NP).
Fusion Partners
Plasmids containing chimaeric heavy chain gene were fused with cells of the J558L mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/20 1/100
Functional Assays 1
  1. 1 This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

References for Chimeric Human IgE anti NP antibody

  1. Neuberger, M.S. et al. (1984) Recombinant antibodies possessing novel effector functions.
    Nature. 312 (5995): 604-8.
  2. Neuberger, M.S. et al. (1985) A hapten-specific chimaeric IgE antibody with human physiological effector function.
    Nature. 314 (6008): 268-70.
  3. Novak, N. et al. (2003) Evidence for a differential expression of the FcepsilonRIgamma chain in dendritic cells of atopic and nonatopic donors.
    J Clin Invest. 111: 1047-56.
  4. Sayers, I. et al. (2004) The importance of Lys-352 of human immunoglobulin E in FcepsilonRII/CD23 recognition.
    J Biol Chem. 279: 35320-5.
  5. Kulka, M. and Metcalfe, D.D. (2004) High-resolution tracking of cell division demonstrates differential effects of TH1 and TH2 cytokines on SCF-dependent human mast cell production in vitro: correlation with apoptosis and Kit expression.
    Blood. 105: 592-9.
  6. Vangelista, L. et al. (2005) Membrane IgE binds and activates Fc epsilon RI in an antigen-independent manner.
    J Immunol. 174: 5602-11.
  7. Sawada, J. et al. (2005) Stem cell factor has a suppressive activity to IgE-mediated chemotaxis of mast cells.
    J Immunol. 174: 3626-32.
  8. Sallmann, E. et al. (2011) High-Affinity IgE Receptors on Dendritic Cells Exacerbate Th2-Dependent Inflammation.
    J Immunol. 187: 164-71.
    9. View The Latest Product References
  9. Xu, D. et al. (2012) RN486, a selective Bruton's tyrosine kinase inhibitor, abrogates immune hypersensitivity responses and arthritis in rodents.
    J Pharmacol Exp Ther. 341 (1): 90-103.
  10. Ferguson, G.D. et al. (2016) A Novel Triazolopyridine-Based Spleen Tyrosine Kinase Inhibitor That Arrests Joint Inflammation.
    PLoS One. 11 (1): e0145705.
  11. Shirley D et al. (2016) Resveratrol preferentially inhibits IgE-dependent PGD2 biosynthesis but enhances TNF production from human skin mast cells.
    Biochim Biophys Acta. pii: S0304-4165(16)00015-5.
  12. Troupin, A. et al. (2016) A Role for Human Skin Mast Cells in Dengue Virus Infection and Systemic Spread.
    J Immunol. 197 (11): 4382-4391.
  13. Bratke, K. et al. (2017) Differential regulation of PD-1 and its ligands in allergic asthma.
    Clin Exp Allergy. 47 (11): 1417-25.
  14. Burton, O.T. et al. (2017) A humanized mouse model of anaphylactic peanut allergy
    J Allergy Clin Immunol139 (1): 314-22.e9.
  15. McHale, C. et al. (2018) Interleukin-6 potentiates FcεRI-induced PGD(2) biosynthesis and induces VEGF from human in situ-matured skin mast cells.
    Biochim Biophys Acta Gen Subj. 1862 (5): 1069-78.
  16. Shan, M. et al. (2018) Secreted IgD Amplifies Humoral T Helper 2 Cell Responses by Binding Basophils via Galectin-9 and CD44.
    Immunity. 49 (4): 709-724.e8.

Functional Assays

RRID
AB_567284

MCA333S

155503 157091 1804

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