The immunoglobulin heavy chain has been produced by the linking of the antigen-binding, variable region genes of a mouse hybridoma to human constant region genes by in vitro DNA recombination procedures. The resulting chimeric antibody is subsequently expressed by the myeloma cell-line J558L after transfection. (The J558L cell-line self secretes a lambda light chain but no heavy chain). Thus a chimeric human IgE antibody specific for NP has been produced.
- Target Species
- Product Form
- Tissue Culture Supernatant - liquid
- Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
- Buffer Solution
- None present
- Preservative Stabilisers
- Hapten, 4-hydroxy-3-nitrophenylacetyl (NP).
- Fusion Partners
- Plasmids containing chimaeric heavy chain gene were fused with cells of the J558L mouse myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Chimeric Human IgE anti NP antibody
|Functional Assays 1
- 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Dialysis cassettes EQU003 are suitable for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Application Based External Images
Product Specific References
References for Chimeric Human IgE anti NP antibody
Neuberger, M.S. et al. (1984) Recombinant antibodies possessing novel effector functions.
Nature. 312 (5995): 604-8.
Sayers, I. et al. (2004) The importance of Lys-352 of human immunoglobulin E in FcepsilonRII/CD23 recognition.
J Biol Chem. 279: 35320-5.
Neuberger, M.S. et al. (1985) A hapten-specific chimaeric IgE antibody with human physiological effector function.
Nature. 314 (6008): 268-70.
Sallmann, E. et al. (2011) High-Affinity IgE Receptors on Dendritic Cells Exacerbate Th2-Dependent Inflammation.
J Immunol. 187: 164-71.
Xu, D. et al. (2012) RN486, a selective Bruton's tyrosine kinase inhibitor, abrogates immune hypersensitivity responses and arthritis in rodents.
J Pharmacol Exp Ther. 341 (1): 90-103.
Vangelista, L. et al. (2005) Membrane IgE binds and activates Fc epsilon RI in an antigen-independent manner.
J Immunol. 174: 5602-11.
Kulka, M. and Metcalfe, D.D. (2004) High-resolution tracking of cell division demonstrates differential effects of TH1 and TH2 cytokines on SCF-dependent human mast cell production in vitro: correlation with apoptosis and Kit expression.
Blood. 105: 592-9.
Novak, N. et al. (2003) Evidence for a differential expression of the FcepsilonRIgamma chain in dendritic cells of atopic and nonatopic donors.
J Clin Invest. 111: 1047-56.
Sawada, J. et al. (2005) Stem cell factor has a suppressive activity to IgE-mediated chemotaxis of mast cells.
J Immunol. 174: 3626-32.
Ferguson, G.D. et al. (2016) A Novel Triazolopyridine-Based Spleen Tyrosine Kinase Inhibitor That Arrests Joint Inflammation.
PLoS One. 11 (1): e0145705.
Shirley D et al. (2016) Resveratrol preferentially inhibits IgE-dependent PGD2 biosynthesis but enhances TNF production from human skin mast cells.
Biochim Biophys Acta. pii: S0304-4165(16)00015-5.
Burton Oliver T. et al. (2016) A humanized mouse model of anaphylactic peanut allergy
Journal of Allergy and Clinical Immunology. Jun 08 [Epub ahead of print]
Troupin, A. et al. (2016) A Role for Human Skin Mast Cells in Dengue Virus Infection and Systemic Spread.
J Immunol. 197 (11): 4382-4391.
Bratke, K. et al. (2017) Differential regulation of PD-1 and its ligands in allergic asthma.
Clin Exp Allergy. 47 (11): 1417-25.
Mchale, C.et al. (2018) Interleukin-6 potentiates FcεRI-induced PGD 2 biosynthesis and induces VEGF from human in situ -matured skin mast cells
Biochimica et Biophysica Acta (BBA) - General Subjects. [Epub ahead of print].