CD95 antibody | C02-6C8

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Mouse anti Human CD95:Biotin

Product Type
Monoclonal Antibody
Clone
C02-6C8
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6273B
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human CD95 antibody, clone C02-6C8, recognizes CD95, also known as TNFRSF6, Apo-1 antigen, apoptosis-mediating surface antigen FAS and FASLG receptor. It is a member of the TNF receptor superfamily and is a death receptor. CD95 is a type I transmembrane glycoprotein which is ubiquitously expressed, but it is particularly abundant in the thymus, liver, heart and kidney tissues (Peter et al. 2015).

Upon binding of its ligand CD95L, CD95 interacts with adaptor proteins to form a death-inducing signaling complex to trigger a cell death signal. However CD95 is also able to promote inflammation, normal and tumor cell growth and migration through other signaling pathways including PI3K, NFκB and JNK MAPKs (Levoin et al. 2020).

The biotinylated Mouse anti Human CD95 antibody, clone C02-6C8 (MCA6273B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human CD95 antibody, clone E06-1A9 (MCA6272GA) as the capture antibody.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
E. coli derived recombinant human full length CD95 (Gln26-Val335)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
P25445
Entrez Gene
FAS
Regulatory
For research purposes only

Applications of CD95 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human CD95 MCA6272GA E 0.1 mg loader

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