CD95 antibody | C02-6C8

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Anti Human CD95 Antibody, clone C02-6C8 thumbnail image 1

Anti Human CD95 Antibody, clone C02-6C8 gallery image 1 — Sandwich ELISA analysis of human CD95 using Mouse anti Human CD95 (MCA6272GA) as a capture reagent and biotinylated Mouse anti Human CD95 (**MCA6273B**) as a detection reagent with purified recombinant CD95 as the antigen. Detection was performed using HRP conjugated streptavidin and substrate.

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Mouse anti Human CD95:Biotin

Product Type: Monoclonal Antibody
Clone: C02-6C8
Isotype: IgG1
Specificity: CD95

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
MCA6273B Datasheet Datasheet SDS SDS	E	0.1 mg		Log in	Get Quote
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Mouse anti Human CD95 antibody, clone C02-6C8, recognizes CD95, also known as TNFRSF6, Apo-1 antigen, apoptosis-mediating surface antigen FAS and FASLG receptor. It is a member of the TNF receptor superfamily and is a death receptor. CD95 is a type I transmembrane glycoprotein which is ubiquitously expressed, but it is particularly abundant in the thymus, liver, heart and kidney tissues (Peter et al. 2015).

Upon binding of its ligand CD95L, CD95 interacts with adaptor proteins to form a death-inducing signaling complex to trigger a cell death signal. However CD95 is also able to promote inflammation, normal and tumor cell growth and migration through other signaling pathways including PI3K, NFκB and JNK MAPKs (Levoin et al. 2020).

The biotinylated Mouse anti Human CD95 antibody, clone C02-6C8 (MCA6273B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human CD95 antibody, clone E06-1A9 (MCA6272GA) as the capture antibody.

Target Species: Human
Product Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Immunogen: E. coli derived recombinant human full length CD95 (Gln26-Val335)
Approx. Protein Concentrations: IgG concentration 0.1 mg/ml
Fusion Partners: Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
ELISA Matched Pair(s): ELISA Matched Pair-Capture Antibody: MCA6272GA
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1.0 ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.