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CD73 antibody | AD2

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Anti Human CD73 Antibody, clone AD2 thumbnail image 1
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Published customer image:
Phycoerythrin conjugated Mouse anti Human CD73 antibody, clone AD2 (MCA6068PE) used to evaluate CD73 expression on bone-marrow derived mesenchymal stem cells by flow cytometry
Image caption:
Chaturvedi CP, Tripathy NK, Minocha E, Sharma A, Rahman K, Nityanand S.
Altered Expression of Hematopoiesis Regulatory Molecules in Lipopolysaccharide-Induced Bone Marrow Mesenchymal Stem Cells of Patients with Aplastic Anemia. Stem Cells Int. 2018 Oct 17;2018:6901761.

From: Insert article citation with space after colon and Hyperlink to PubMed record.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human CD73:FITC

Product Type
Monoclonal Antibody
Clone
AD2
Isotype
IgG1
Specificity
CD73

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Product Code Applications Pack Size List Price Your Price Qty
MCA6068F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/0.5ml loader
List Price Your Price
loader

Mouse anti Human CD73, clone AD2 recognizes CD73 also known as ecto-5′-nucleotidase. CD73 is a enzyme that catalyzes the coversion of AMP to adenosine. CD73 expression has been detected on subsets of T cells and B cells, follicular dendritic cells, endothelial cells and mesenchymal stem cells. CD73 has been implicated in T cell activation and lymphocyte adhesion.

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Immunogen
Pre-B leukemia cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/2 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
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List Price Your Price
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Description Mouse IgG1 Negative Control:FITC

References for CD73 antibody

  1. Krüger, K.H. et al. (1991) Expression of ecto-5′-nucleotidase (CD73) in normal mammary gland and in breast carcinoma.
    Br J Cancer. 63 (1): 114-8.
  2. Yoo, H.S. et al. (2013) Mesenchymal Stem Cell Lines Isolated by Different Isolation Methods Show Variations in the Regulation of Graft-versus-host Disease.
    Immune Netw. 13 (4): 133-40.
  3. Terp, M.G. et al. (2013) Anti-human CD73 monoclonal antibody inhibits metastasis formation in human breast cancer by inducing clustering and internalization of CD73 expressed on the surface of cancer cells.
    J Immunol. 191 (8): 4165-73.
  4. Chaturvedi, C.P. et al. (2018) Altered Expression of Hematopoiesis Regulatory Molecules in Lipopolysaccharide-Induced Bone Marrow Mesenchymal Stem Cells of Patients with Aplastic Anemia.
    Stem Cells Int. 2018: 6901761.
  5. GarikipatiV, N.S. et al. (2018) Isolation and characterization of mesenchymal stem cells from human fetus heart.
    PLoS One. 13 (2): e0192244.
  6. Di Paola, A. et al. (2021) Eltrombopag in paediatric immune thrombocytopenia: Iron metabolism modulation in mesenchymal stromal cells.
    Br J Haematol. Dec 28 [Epub ahead of print].
  7. Tiraihi, T. et al. (2023) A Sequential Culturing System for Generating Epithelial-Like Stem Cells from Human Mesenchymal Stem Cells Derived from Adipose Tissue
    Cell Tissue Biol. 17 (6): 639-52.
  8. Bami, M. et al. (2020) Superiority of synovial membrane mesenchymal stem cells in chondrogenesis, osteogenesis, myogenesis and tenogenesis in a rabbit model.
    Injury. 51 (12): 2855-65.
    9. View The Latest Product References

UniProt
Q6NZX3

MCA6068F

156369 163228 168139

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