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CD73 antibody | AD2

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Mouse anti Human CD73:FITC

Product Type
Monoclonal Antibody
Clone
AD2
Isotype
IgG1
Specificity
CD73

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Product Code Applications Pack Size List Price Your Price Qty
MCA6068F
Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/0.5ml loader
List Price Your Price
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Anti Human CD73 Antibody, clone AD2 thumbnail image 1 Anti Human CD73 Antibody, clone AD2 thumbnail image 2
Anti Human CD73 Antibody, clone AD2 gallery image 1
Published customer image:
Phycoerythrin conjugated Mouse anti Human CD73 antibody, clone AD2 (MCA6068PE) used to evaluate CD73 expression on human tumor derived mesenchymal stromal cells by flow cytometry.
Image caption:
Immune phenotype of MSC is not markedly affected by SAHA treatment. (A) Flow cytometric analysis of cell surface markers on T-MSC. T-MSC were treated with SAHA for two days. Isotype controls are depicted in solid lines, and CD73, CD90, and CD105 stainings are depicted in filled histograms. Numbers show the mean fluorescence intensity (MFI). The expression of the surface marker CD73 moderately increased with increasing SAHA concentrations. Expression of CD90 and CD105 was not markedly affected. (B) BM-MSC showed an increased expression of CD90, a reduced expression of CD105 with increasing SAHA concentrations, and an unaltered expression of CD73 up to 1 μM SAHA. SAHA: suberoylanilide hydroxamic acid.

From: Kruchen, A.; Johann, P.-D.; Rekowski, L.; Müller, I.
Epigenetic Modification of Mesenchymal Stromal Cells Derived from Bone Marrow and Embryonal Tumors to Facilitate Immunotherapeutic Approaches in Pediatric Malignancies.
Curr Issues Mol Biol. 2023, 45, 2121-35.
doi: 10.3390/cimb45030136
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Human CD73 Antibody, clone AD2 gallery image 2
Published customer image:
FITC conjugated Mouse anti Human CD73 antibody, clone AD2 (MCA6068F) used for the evaluation of phenotype of cultured mesenchymal stem cells by flow cytometry.
Image caption:
The maintenance of stemness of bare hMSCs and ECM-hMSCs. C) Stemness analysis to confirm stem cell characteristics by flow cytometry analysis.

From: Park CY, Choi K, Kim YJ, Chung S, Choi JS, Park SJ, Kim CH.
External-Force-Offset Effects of ECM Coating Layers on hMSCs Subjected to External Physical Force.
Biomater Res. 2025 Oct 3;29:0265.
doi: 10.34133/bmr.0265.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Mouse anti Human CD73, clone AD2 recognizes CD73 also known as ecto-5′-nucleotidase. CD73 is a enzyme that catalyzes the coversion of AMP to adenosine. CD73 expression has been detected on subsets of T cells and B cells, follicular dendritic cells, endothelial cells and mesenchymal stem cells. CD73 has been implicated in T cell activation and lymphocyte adhesion.

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Immunogen
Pre-B leukemia cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

This product is shipped at ambient temperature.
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/2 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul

How to Use the Spectraviewer

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  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
List Price Your Price
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Description Mouse IgG1 Negative Control:FITC

References for CD73 antibody

  1. Krüger, K.H. et al. (1991) Expression of ecto-5′-nucleotidase (CD73) in normal mammary gland and in breast carcinoma.
    Br J Cancer. 63 (1): 114-8.
  2. Yoo, H.S. et al. (2013) Mesenchymal Stem Cell Lines Isolated by Different Isolation Methods Show Variations in the Regulation of Graft-versus-host Disease.
    Immune Netw. 13 (4): 133-40.
  3. Terp, M.G. et al. (2013) Anti-human CD73 monoclonal antibody inhibits metastasis formation in human breast cancer by inducing clustering and internalization of CD73 expressed on the surface of cancer cells.
    J Immunol. 191 (8): 4165-73.
  4. Chaturvedi, C.P. et al. (2018) Altered Expression of Hematopoiesis Regulatory Molecules in Lipopolysaccharide-Induced Bone Marrow Mesenchymal Stem Cells of Patients with Aplastic Anemia.
    Stem Cells Int. 2018: 6901761.
  5. GarikipatiV, N.S. et al. (2018) Isolation and characterization of mesenchymal stem cells from human fetus heart.
    PLoS One. 13 (2): e0192244.
  6. Di Paola, A. et al. (2021) Eltrombopag in paediatric immune thrombocytopenia: Iron metabolism modulation in mesenchymal stromal cells.
    Br J Haematol. Dec 28 [Epub ahead of print].
  7. Tiraihi, T. et al. (2023) A Sequential Culturing System for Generating Epithelial-Like Stem Cells from Human Mesenchymal Stem Cells Derived from Adipose Tissue
    Cell Tissue Biol. 17 (6): 639-52.
  8. Bami, M. et al. (2020) Superiority of synovial membrane mesenchymal stem cells in chondrogenesis, osteogenesis, myogenesis and tenogenesis in a rabbit model.
    Injury. 51 (12): 2855-65.
    9. View The Latest Product References
  9. Park, C.Y. et al. (2025) External-Force-Offset Effects of ECM Coating Layers on hMSCs Subjected to External Physical Force.
    Biomater Res. 29: 0265.

UniProt
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MCA6068F

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