CD62P antibody | A05-6A1

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Mouse anti Human CD62P:Biotin

Product Type
Monoclonal Antibody
Clone
A05-6A1
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA6275B
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human CD62P antibody, clone A05-6A1, recognizes CD63P, also known as P-Selectin, granule membrane protein 140, LECAM and PADGEM. CD62P is a member of the Selectin family of proteins which are transmembrane Ca2+-dependent lectins that mediate adhesion of leukocytes, platelets and the endothelium during inflammation and immune surveillance (McEver 2015).

CD62P is expressed by megakaryocytes and endothelial cells and is stored in membranes of α-granules of platelets and Weibel-Palade bodies of endothelial cells. In humans CD62P can also be secreted as a molecule lacking the transmembrane domain. Increased levels of soluble CD62P in peripheral blood has been documented in a variety of diseases including heart disease, diabetes, hypertension and atrial fibrillation (Polgar et al. 2005).

The biotinylated Mouse anti Human CD62P antibody, clone A05-6A1 (MCA6275B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human CD62P antibody, clone A01-2H1 (MCA6274GA) as the capture antibody.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
HEK293 derived recombinant human full length CD62P (Met1- Ala771)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
P16109
Entrez Gene
SELP
GO Terms
GO:0001530 lipopolysaccharide binding
GO:0002576 platelet degranulation
GO:0005887 integral to plasma membrane
GO:0005615 extracellular space
GO:0005624 membrane fraction
GO:0005625 soluble fraction
GO:0008201 heparin binding
GO:0050829 defense response to Gram-negative bacterium
GO:0009897 external side of plasma membrane
GO:0010572 positive regulation of platelet activation
GO:0030168 platelet activation
GO:0031088 platelet dense granule membrane
GO:0031092 platelet alpha granule membrane
GO:0032496 response to lipopolysaccharide
GO:0033691 sialic acid binding
GO:0042806 fucose binding
GO:0043208 glycosphingolipid binding
GO:0070492 oligosaccharide binding
Regulatory
For research purposes only

Applications of CD62P antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human CD62P MCA6274GA E 0.1 mg loader

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