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CD324 antibody | 67A4

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Anti Human CD324 Antibody, clone 67A4 thumbnail image 1 Anti Human CD324 Antibody, clone 67A4 thumbnail image 2 Anti Human CD324 Antibody, clone 67A4 thumbnail image 3
Anti Human CD324 Antibody, clone 67A4 gallery image 1
Surface staining of HT-29 / SP2 cells with Mouse anti Human CD324:APC (MCA6135APC)
Anti Human CD324 Antibody, clone 67A4 gallery image 2
Surface staining of HT-29 cells with Mouse anti Human CD324:FITC (MCA6135F)
Anti Human CD324 Antibody, clone 67A4 gallery image 3
Surface staining of HT-29 / SP2 cells with Mouse anti Human CD324:RPE (MCA6135PE)

Mouse anti Human CD324:APC

Product Type
Monoclonal Antibody
Clone
67A4
Isotype
IgG1
Specificity
CD324

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Product Code Applications Pack Size List Price Your Price Qty
MCA6135APC
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
List Price Your Price
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Mouse anti Human CD324, clone 67A4 recognizes CD324 also known as E-Cadherin, a transmembrane glycoprotein which mediates calcium dependant cell adhesion and cell junction formation.

The extracellular domain mediates a Ca2+ dependant homophillic interaction with a cadherin molecule on the surface of a neighboring cell. This leads to the formation of tight junctions which assist in blocking the movement of cells and facilitating cell-cell interactions (Gloushankova et al. 2017).

The cytoplasmic domain is able to interact with catenins which link the CD324 to the actin cytoskeleton which help mediate downstream signaling events growth inhibitory signals to prevent uncontrolled tissue growth during embryonic development, tissue regeneration, wound healing. Loss of CD324 expression has been associated with tumor progression and metastasis. It is thought that metastasis is more likely to occur due to the loss of a maintained stable physical link between tumor cells (Mendonsa et al. 2018).

This clone 67A4 has been used in immunocytochemistry experiments to examine the localization of CD324 on medullary epithelial cells (Kutleša et al. 2002).

Target Species
Human
Product Form
Purified IgG conjugated to Allophycyanin (APC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Immunogen
T-47D cells
Purity
>95% by SDS PAGE
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
APC 650 661
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:APC MCA928APC F 100 Tests
>
List Price Your Price
Description Mouse IgG1 Negative Control:APC

References for CD324 antibody

  1. Mendonsa, A.M. et al. (2018) E-cadherin in contact inhibition and cancer.
    Oncogene. 37 (35): 4769-4780.
  2. Gloushankova, N.A. et al. (2017) Cadherin-mediated cell-cell interactions in normal and cancer cells.
    Tissue Barriers. 5 (3): e1356900.
  3. Kutlesa, S. et al. (2002) E-cadherin-mediated interactions of thymic epithelial cells with CD103+ thymocytes lead to enhanced thymocyte cell proliferation.
    J Cell Sci. 115 (Pt 23): 4505-15.

Synonyms
E-Cadherin
UniProt
P12830
Entrez Gene
CDH1
GO Terms
GO:0016021 integral to membrane
GO:0050839 cell adhesion molecule binding
GO:0005913 cell-cell adherens junction
GO:0005925 focal adhesion
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007156 homophilic cell adhesion
GO:0015629 actin cytoskeleton
GO:0016235 aggresome
GO:0016328 lateral plasma membrane
GO:0016342 catenin complex
GO:0016563 transcription activator activity
GO:0022408 negative regulation of cell-cell adhesion
GO:0034329 cell junction assembly
GO:0034332 adherens junction organization
GO:0042993 positive regulation of transcription factor import into nucleus
GO:0043296 apical junction complex
GO:0045295 gamma-catenin binding
GO:0048471 perinuclear region of cytoplasm
GO:0050776 regulation of immune response
GO:0071285 cellular response to lithium ion
GO:0071681 cellular response to indole-3-methanol

MCA6135APC

149889 163815

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