CD258 antibody | B01-9B3

Mouse anti Human CD258:Biotin

Product Type
Monoclonal Antibody
Clone
B01-9B3
Isotype
IgG2a
Specificity
CD258

Product Code Applications Pack Size List Price Your Price Qty
MCA6281B
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader
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Mouse anti Human CD258 antibody, clone B01-9B3, recognizes CD258 also known as TNFSF14, Herpes virus entry mediator and LIGHT. CD258 is the ligand of the receptor TNFRSF14, which is the cell surface receptor that the Herpes virus uses to attach to the cell surface and to enter into the cell, it is also a ligand of lymphotoxin-β receptor (Ware & Sedý 2011). Signaling events initiated upon binding of CD258 to these receptors is cell dependent. However, the responses are often related to immune system stimulation including the production of various cytokines, adhesion molecules that control the migration and positioning of dendritic cells and lymphocytes (Skeate et al. 2020, Tang et al. 2016).

CD258 is primarily expressed by activated T cells, activated natural killer cells and immature dentritic cells. It is initially synthesized as a type II transmembrane protein, which can be proteolytically cleaved to release a soluble bioactive form (Ware & Sedý 2011).

The biotinylated Mouse anti Human CD258 antibody, clone B01-9B3 (MCA62781B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human CD258 antibody, clone D04-8A4 (MCA6280GA) as the capture antibody.

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
E. coli derived recombinant human CD258 (Leu61-Val240)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Human CD258 MCA6280GA E 0.1 mg loader
List Price Your Price
loader
Description Mouse anti Human CD258

Synonyms
TNFSF14
UniProt
O43557
Entrez Gene
TNFSF14
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0005615 extracellular space
GO:0005737 cytoplasm
GO:0006917 induction of apoptosis
GO:0006955 immune response
GO:0008588 release of cytoplasmic sequestered NF-kappaB
GO:0042098 T cell proliferation
GO:0043027 caspase inhibitor activity
GO:0043029 T cell homeostasis
View more products with CD258 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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