CD127 antibody | AbD11590

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Anti Human CD127 Antibody, clone AbD11590 thumbnail image 1

Anti Human CD127 Antibody, clone AbD11590 thumbnail image 2

Anti Human CD127 Antibody, clone AbD11590 gallery image 1 — Human peripheral blood lymphocytes stained with Human anti Human CD127 (**HCA144**) followed by Mouse anti Strep-tag (MCA2488) and FITC conjugated Rabbit F(ab′)₂ anti Mouse IgG (STAR9B).

Anti Human CD127 Antibody, clone AbD11590 gallery image 2 — Acetone fixed frozen human tonsil stained with Human anti Human CD127 (**HCA144**) followed by Mouse anti Strep-tag (MCA2488).

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Human anti Human CD127

Product Type: Monoclonal Antibody
Clone: AbD11590
Isotype: HuCAL Fab bivalent
Specificity: CD127

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
HCA144 Datasheet Datasheet SDS SDS	C F *	0.1 mg		Log in	Get Quote
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Human anti Human CD127 antibody, clone AbD11590 recognizes the alpha chain of the human interleukin-7 receptor (IL-7R), which is also known as CD127. CD127 is a ~65-75 kDa transmembrane glycoprotein that associates with the interleukin-2 receptor gamma chain to form the functional high affinity IL-7 receptor. CD127 is expressed on lymphoid precursors in the bone marrow, pro-B cells, a subpopulation of thymocytes, T-cells and monocytes. CD127 is the specific receptor for IL-7 and the IL-7 receptor plays a critical role in lymphoid development.

Target Species: Human
Product Form: A bivalent human recombinant Fab selected from the HuCAL phage display library, expressed in E.coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a DYKDDDDK tag and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
Preparation: StrepTactin affinity chromatography
Source: E.coli
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Recombinant human IL-7 R alpha/CD127/Fc chimera
Approx. Protein Concentrations: Ig concentration 0.5 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry ¹	1/10	1/200
Immunohistology - Frozen
Western Blotting

¹HCA144 has been demonstrated to function in Flow Cytometry.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice: Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
Histology Positive Control Tissue: Human tonsil

Description	Product Code	Applications	Pack Size	Your Price
Rabbit anti DYKDDDDK Tag:HRP	AHP1074P	E WB	0.1 mg	Log in	Get Quote
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Description	Rabbit anti DYKDDDDK Tag:HRP
Mouse anti Strep-Tag Immo	MCA2488	E	0.1 mg	Log in	Get Quote
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Description	Mouse anti Strep-Tag Immo

Synonyms: IL-7R
RRID: AB_10612595
UniProt: P16871
Entrez Gene: IL7R
Licensed Use: For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.