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CCL8 antibody | E09-8A1

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Anti Human CCL8 Antibody, clone E09-8A1 thumbnail image 1
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Sandwich ELISA analysis of human CCL8 using Mouse anti Human CCL8 (MCA6266GA) as a capture reagent and biotinylated Mouse anti Human CCL8 (MCA6267B) as a detection reagent with purified recombinant CCL8 as the antigen. Detection was performed using HRP conjugated streptavidin and substrate.

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Mouse anti Human CCL8:Biotin

Product Type
Monoclonal Antibody
Clone
E09-8A1
Isotype
IgG2a
Specificity
CCL8

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Product Code Applications Pack Size List Price Your Price Qty
MCA6267B
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader

Mouse anti Human CCL8 antibody, clone E09-8A1, recognizes C-C motif chemokine-8 (CCL8) also known as HC14, monocyte chemoattractant protein 2, monocytes chemotactic protein 2 and small-inducible cytokine A8. CCL8 is a member of the C-C chemokine subfamily and is an agonist for C-C chemokine receptor type 2 and CCR5 (Ge et al. 2017). Through these receptors, CCL8 is involved in the immune response by attracting monocytes, T-lymphocytes, natural killer cells, basophils, mast cells and eosinophils (Farmaki et al. 2020). Proteolytic cleavage of CCL8 to CCL8 (6-76) converts the chemokine into a potent inhibitor of C-C chemokine-induced chemotaxis (Proost et al. 1998).

The biotinylated Mouse anti Human CCL8 antibody, clone E09-8A1 (MCA6267B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human CCL8 antibody, clone E07-8G5 (MCA6266GA) as the capture antibody.

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
E. coli derived recombinant human full length CCL8 (Gln24-Pro99)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
ELISA Matched Pair(s)
ELISA Matched Pair-Capture Antibody: MCA6266GA ELISA Antigen-Positive Control: PHP297
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Human CCL8 MCA6266GA E 0.1 mg loader
List Price Your Price
loader
Description Mouse anti Human CCL8
Recombinant Human CCL8 PHP297 E 10 µg
List Price Your Price
Description Recombinant Human CCL8

Synonyms
MCP-2
UniProt
P80075
Entrez Gene
CCL8
GO Terms
GO:0009615 response to virus
GO:0004871 signal transducer activity
GO:0005615 extracellular space
GO:0006816 calcium ion transport
GO:0006887 exocytosis
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007267 cell-cell signaling
GO:0008009 chemokine activity
GO:0008201 heparin binding

MCA6267B

100005464

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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