CD45 antibody | IH-1
Mouse anti Guinea pig CD45 antibody, clone IH-1 (MCA1130) used for the evaluation of CD45 expression on cultured bone marrow derived mesenchymal stem cells by flow cytometry.
Image caption:
Validation of MSC properties. a BM-MSCs were harvested and cultured from guinea pigs’ bone marrow from healthy (sham-exposed) or CS-exposed individuals. b Cell characterization was determined by plastic adhesion, colony forming capacity, and morphology. c Purity was checked by flow cytometric analysis of CD45 expression in BM-MSCs and compared with whole blood. Fluorescence intensity of the no-antibody (left panel), the secondary antibody control (middle panel) and fluorescence intensity in the presence of the CD45 specific antibody (right panel) was measured. The lower panel shows gene expression analysis of positive (CD105, CD90, CD73) and negative (CD11b, CD31, CD34) MSC markers and β-Actin. M: molecular weight; LS: lung tissue lysate. d Adipogenic and oesteogenic differentiation. Cells were incubated for 3 weeks in either induction or normal growth medium before von-Kossa and Alizarin red staining for calcium deposits or oil red to identify lipidic vacuoles
From: Tura-Ceide O, Lobo B, Paul T, Puig-Pey R, Coll-Bonfill N, García-Lucio J, Smolders V, Blanco I, Barberà JA, Peinado VI.
Cigarette smoke challenges bone marrow mesenchymal stem cell capacities in guinea pig.
Respir Res. 2017 Mar 23;18(1):50.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Guinea Pig CD45:FITC
- Product Type
- Monoclonal Antibody
- Clone
- IH-1
- Isotype
- IgG1
- Specificity
- CD45
Antibody Quality and Performance Guarantee
| Mouse anti Guinea Pig CD45 antibody, clone IH-1 reacts with CD45 on all leucocytes. Mouse anti Guinea Pig CD45 antibody, clone IH-1 immunoprecipitates proteins of M.Wt. 220,000, 210,000 and 195,000 and 180,000 from lymph node lymphocytes and proteins of M.Wt 195,000, 180,000 and 170,000 from thymocytes. |
- Target Species
- Guinea Pig
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Guinea pig leukemic B lymphocyte L2C cells.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/10 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control:FITC | ||||||
References for CD45 antibody
-
Hart, I.J. et al. (1992) Subpopulations of guinea-pig T lymphocytes defined by isoforms of the leucocyte common antigen.
Immunology. 77 (3): 377-84. -
Wang, Y. et al. (2010) Local host response to chlamydial urethral infection in male guinea pigs.
Infect Immun. 78: 1670-81. -
Lacy, H.M. et al. (2011) Essential role for neutrophils in pathogenesis and adaptive immunity in Chlamydia caviae ocular infections.
Infect Immun. 79 (5): 1889-97. -
Ishikawa, M. et al. (2017) Transplantation of neurons derived from human iPS cells cultured on collagen matrix into guinea-pig cochleae.
J Tissue Eng Regen Med. 11 (6): 1766-78. -
Tura-Ceide, O. et al. (2017) Cigarette smoke challenges bone marrow mesenchymal stem cell capacities in guinea pig.
Respir Res. 18 (1): 50.
- RRID
- AB_566763
MCA1130F
0214
154728
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