CD94 antibody | 8H10
Mouse anti Dog CD94:Alexa Fluor® 488
- Product Type
- Monoclonal Antibody
- Clone
- 8H10
- Isotype
- IgG1
- Specificity
- CD94
Antibody Quality and Performance Guarantee
MCA6400A488
| Mouse anti Dog CD94 antibody, clone 8H10 recognizes canine CD94, also known as KLRD1 (Killer cell lectin like receptor D1). CD94 is expressed on natural killer (NK) cells and on natural killer-like T (NKT) cells. In humans, a range of receptors that can identify and modulate NK cell function have been well described. Among them CD94 which associates with members from the NKG2 family to form activating or inhibitory heterodimers. It is not established whether the same interaction happens in dogs. Canine NK cells remain poorly characterized with mouse anti dog CD94 being one of few markers available to detect them (Graves et al. 2019). Mouse anti dog CD94 antibody, clone 8H10 will detect approx. 7.7% (NK cell range in dogs: 2.5% – 15%) (Graves et al. 2019, Kisseberth and Lee, 2021) of freshly isolated canine PBMC and has been used for magnetic bead cell isolation (Graves et al. 2019). |
- Target Species
- Dog
- Product Form
- Purified IgG conjugated to Alexa Fluor®488 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- CD94-muIgG2a fusion protein
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the P3X63Ag8.653 myeloma cell line
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®488 495 519 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA928A488 | F | 100 Tests/1ml |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control:Alexa Fluor® 488 | ||||||
Antibody Characterization Reference
-
Graves, S.S. et al. (2019) Development and characterization of a canine-specific anti-CD94 (KLRD-1) monoclonal antibody.
Vet Immunol Immunopathol. 211: 10-18.
- UniProt
- Q38HS3
- Entrez Gene
- KLRD1
- GO Terms
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0005529 sugar binding
MCA6400A488
162565
163671
165388
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