CD44 antibody | YKIX337.8.7

Rat anti Dog CD44:Alexa Fluor® 488

Product Type
Monoclonal Antibody
Clone
YKIX337.8.7
Isotype
IgG2a
Specificity
CD44

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MCA1041A488
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Rat anti Dog CD44 antibody, clone YKIX337.8.7 recognizes canine CD44, also known as H-CAM, a single-pass type 1 membrane of approximately 90 kDa expressed by most leucocytes and epithelial cells. CD44 expression is markedly increased upon cell activation (Alldinger et al. 2000).

CD44 is involved in cell-cell, cell adhesion and cell migration and is the principal cellular receptor for hyaluronate via it's LINK domain, additionally CD44 interacts with other ligands including collagens and metalloproteinases.

Altered CD44 expression is detected in many forms of invasive and metastatic cancer, CD44 expression has been observed on canine mammary and melanocytic tumors (Serra et al. 2004).

Target Species
Dog
Species Cross-Reactivity
Target SpeciesCross Reactivity
Raccoon
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor 488 - liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% sodium azide (NaN3).
1% bovine serum albumin.
Immunogen
Concanavilin A activated Canine T cells.
Approx. Protein Concentrations
IgG concentration 0.05mg/ml.
Fusion Partners
Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3.myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®488 495 519
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/20
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

References for CD44 antibody

  1. Cobbold, S. & Metcalfe,S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).
    Tissue Antigens. 43 (3): 137-54.
  2. Stein, V.M. et al. (2008) Immunophenotypical characterization of monocytes in canine distemper virus infection.
    Vet Microbiol. 131:237-46.
  3. Salvatierra, A. et al. (2001) Antithrombin III prevents early pulmonary dysfunction after lung transplantation in the dog.
    Circulation. 104: 2975-80.
  4. Sanchez, M.A. et al. (2004) Organ-specific immunity in canine visceral leishmaniasis: analysis of symptomatic and asymptomatic dogs naturally infected with Leishmania chagasi.
    Am J Trop Med Hyg. 70: 618-24.
  5. Stein, V.M. et al. (2004) Characterization of canine microglial cells isolated ex vivo.
    Vet Immunol Immunopathol. 99: 73-85.
  6. Heinrich, F. et al. (2015) Immunophenotyping of immune cell populations in the raccoon (Procyon lotor).
    Vet Immunol Immunopathol. 168 (3-4): 140-6.
  7. Bearden, R.N. et al. (2017) In-vitro characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: a donor-matched comparative study.
    Stem Cell Res Ther. 8 (1): 218.
  8. Trindade, A.B. et al. (2017) Mesenchymal-like stem cells in canine ovary show high differentiation potential.
    Cell Prolif. 50 (6)Oct 08 [Epub ahead of print].
  9. View The Latest Product References
  10. Muir, P. et al. (2016) Autologous Bone Marrow-Derived Mesenchymal Stem Cells Modulate Molecular Markers of Inflammation in Dogs with Cruciate Ligament Rupture.
    PLoS One. 11 (8): e0159095.
  11. Salinas Tejedor, L. et al. (2015) Mesenchymal stem cells do not exert direct beneficial effects on CNS remyelination in the absence of the peripheral immune system.
    Brain Behav Immun. 50: 155-65.
  12. Heinrich, F. et al. (2015) Passage-dependent morphological and phenotypical changes of a canine histiocytic sarcoma cell line (DH82 cells).
    Vet Immunol Immunopathol. 163 (1-2): 86-92.
  13. Wijekoon, H.M.S. et al. (2017) Differentiation potential of synoviocytes derived from joints with cranial cruciate ligament rupture and medial patella luxation in dogs.
    Res Vet Sci. 114: 370-7.
  14. Hansmann, F. et al. (2018) Beneficial and detrimental impact of transplanted canine adipose-derived stem cells in a virus-induced demyelinating mouse model.
    Vet Immunol Immunopathol. 202: 130-40.
  15. Elshafae, S.M. et al. (2017) The Effect of a Histone Deacetylase Inhibitor (AR-42) on Canine Prostate Cancer Growth and Metastasis.
    Prostate. 77 (7): 776-93.
  16. Gouveia, G.M. et al. (2013) Analysis of cancer stem cells in dog’s mammary neoplasias.
    Braz J Vet Med, 35(3), 229-35.

Synonyms
H-CAM
PGP-1
RRID
AB_10926391
UniProt
Q28284
Entrez Gene
CD44
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0005540 hyaluronic acid binding
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