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CD18 antibody | CA1.4E9

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Anti Dog CD18 Antibody, clone CA1.4E9 thumbnail image 1 Anti Dog CD18 Antibody, clone CA1.4E9 thumbnail image 2 Anti Dog CD18 Antibody, clone CA1.4E9 thumbnail image 3 Anti Dog CD18 Antibody, clone CA1.4E9 thumbnail image 4 Anti Dog CD18 Antibody, clone CA1.4E9 thumbnail image 5
Anti Dog CD18 Antibody, clone CA1.4E9 gallery image 1
Figure A. RPE conjugated Mouse anti Canine CD21 (MCA1781PE) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. RPE conjugated Mouse anti Canine CD21 (MCA1781PE) and Alexa Fluor 647 conjugated Mouse anti Canine CD18 (MCA1780A647). All experiments performed on red cell lysed canine blood gated on lymphocytes in the presence of 10% canine serum. Data acquired on the ZE5 Cell Analyzer.
Anti Dog CD18 Antibody, clone CA1.4E9 gallery image 2
Published customer image:
Mouse anti Dog CD18 antibody, clone CA1.4E9 (MCA1780GA) used for the evaluation of CD18 expression on alveolar macrophages from hooded seal (Cystophora cristata) using flow cytometry.
Image caption:
Expression of membrane markers. The expression of CD14 and CD18 on hooded seal PBMC and BAL cells by flow cytometric analysis. PBMCs (left) were divided into monocyte and lymphocyte gates, while BAL cells (right) were broadly gated to encompass all cells of likely macrophage appearance. Only cells classified as viable (using Live/Dead fixable far red stain) were routed to analysis. The respective cell populations were analyzed for CD14 and CD18 (dark grey) or unstained cells (open histograms). For CD18 an indirect staining technique was used, and secondary antibodies alone are depicted in light grey histograms; the resulting dimly positive staining were assumed to be due to unspecific binding, possibly due to Fc receptor cross-reactivity (CD14; cluster of differentiation 14, CD18; cluster of differentiation 18, PBMC; peripheral blood mononuclear cell).

From: Larsen AK, Nymo IH, Boysen P, Tryland M, Godfroid J (2013)
Entry and Elimination of Marine Mammal Brucella spp. by Hooded Seal (Cystophora cristata) Alveolar Macrophages In Vitro.
PLoS ONE 8(7): e70186.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Dog CD18 Antibody, clone CA1.4E9 gallery image 3
Published customer image:
Mouse anti Canine CD18 antibody, clone CA1.4E9 (MCA1780GA) used for the evaluation of CD18 expression on alveolar macrophages from hooded seal (Cystophora cristata) using immunofluorescence microscopy.
Image caption:
Macrophage identification. Adherent BAL cells cultured for 5–7 days were examined for the expression of membrane markers by the use of mAbs. Representative confocal micrographs showing BAL cells immunostained for CD14 (red) and CD18 (green). Nuclei are visualized by DRAQ5 (blue). Pictures are taken of wet samples using a 40X 1.2NA water immersion lens (mAbs; monoclonal antibodies).

From: Larsen AK, Nymo IH, Boysen P, Tryland M, Godfroid J (2013)
Entry and Elimination of Marine Mammal Brucella spp. by Hooded Seal (Cystophora cristata) Alveolar Macrophages In Vitro.
PLoS ONE 8(7): e70186.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Dog CD18 Antibody, clone CA1.4E9 gallery image 4
Figure A. A647 conjugated Mouse anti Canine CD18 (MCA1780A647) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. A647 conjugated Mouse anti Canine CD18 (MCA1780A647) and RPE conjugated Mouse anti Canine CD21 (MCA1781PE). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Anti Dog CD18 Antibody, clone CA1.4E9 gallery image 5
Figure A. RPE conjugated Mouse anti Canine CD21 (MCA1781PE) and A647 conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. RPE conjugated Mouse anti Canine CD21 (MCA1781PE) and A647 conjugated Mouse anti Canine CD18 (MCA1780A647). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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Mouse anti Dog CD18:Alexa Fluor®647

Product Type
Monoclonal Antibody
Clone
CA1.4E9
Isotype
IgG1
Specificity
CD18

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Product Code Applications Pack Size List Price Your Price Qty
MCA1780A647
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F 100 Tests/1ml loader
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Mouse anti Dog CD18 antibody, clone CA1.4E9 recognizes the canine CD18 cell surface antigen, also known as the b2 integrin.

The tissue and cellular distribution of CD18 in canine tissue closely follows that observed for humans (Moore et al. 1990) CD18 is expressed by virtually all leucocytes, but more strongly upon monocytes and granulocytes than on lymphocytes. The cross reactivity patterns of Mouse anti Canine CD18, clone CA1.4E9 further indicate that the antibody clone recognizes an epitope common to a number of mammalian species.

Immunoprecipitation experiments using detergent lysates of iodinated peripheral blood leukocytes indicate that clone Ca1.4E9 immunoprecipitates the common 95 kDa beta 2 integrin chain (CD18) along with the non-covalently associated alpha chains at 180 kDa (CD11a ), 165 kDa (CD11b) and 150 kDa (CD11c) (Moore et al. 1990). Of note is the lack of precipitation of the integrin alpha D chain (CD11d), this is likely due to the almost complete absence of CD11d expression on peripheral blood lymphocytes, in contrast to expression in the splenic red pulp (Fry et al. 2003).

High CD18 expression is a common feature of lymphoma and hyperplasia in dogs (Caniatti et al. 1996)

Target Species
Dog
Species Cross-Reactivity
Target SpeciesCross Reactivity
Horse
Pig
Human
Bovine
Cat
Mustelid Expected from Sequence
Mink
Hooded Seal
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 F 100 Tests/1ml loader
List Price Your Price
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Description Mouse IgG1 Negative Control:Alexa Fluor® 647

Source Reference

  1. Moore, P.F. et al. (1990) Canine leukocyte integrins: characterization of a CD18 homologue.
    Tissue Antigens. 36 (5): 211-20.

References for CD18 antibody

  1. Danilenko, D.M. et al. (1992) Canine leukocyte cell adhesion molecules (leuCAMS): characterization of the CD11/CD18 family.
    Tissue Antigens 40: 13-21.
  2. Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species.
    Vet Immunol Immunopathol. 64 (1): 1-13.
  3. Leite, F. et al. (2000) Recombinant bovine interleukin-1beta amplifies the effects of partially purified Pasteurella haemolytica leukotoxin on bovine neutrophils in a beta(2)-integrin-dependent manner.
    Infect Immun. 68: 5581-6.
  4. McDonough, S.P. and Moore, P.F. (2000) Clinical, hematologic, and immunophenotypic characterization of canine large granular lymphocytosis
    Vet Pathol. 37: 637-46.
  5. Comazzi, S. et al. (2006) Flow cytometric patterns in blood from dogs with non-neoplastic and neoplastic hematologic diseases using double labeling for CD18 and CD45.
    Vet Clin Pathol. 35 (1): 47-54.
  6. Bauer, T.R. Jr. et al. (2006) Correction of the disease phenotype in canine leukocyte adhesion deficiency using ex vivo hematopoietic stem cell gene therapy.
    Blood. 108: 3313-20.
  7. Comazzi, S. et al. (2006) Flow cytometric expression of common antigens CD18/CD45 in blood from dogs with lymphoid malignancies: a semi-quantitative study.
    Vet Immunol Immunopathol. 112 (3-4): 243-52.
  8. Lecchi, C. et al. (2008) Bovine alpha-1 acid glycoprotein can reduce the chemotaxis of bovine monocytes and modulate CD18 expression.
    Vet Res. 39: 50.
    9. View The Latest Product References
  9. Mortarino, M. et al. (2010) Identification of suitable endogenous controls and differentially expressed microRNAs in canine fresh-frozen and FFPE lymphoma samples.
    Leuk Res. 34: 1070-7.
  10. Donahue, R.E. et al. (2011) Leukocyte integrin activation mediates transient neutropenia after G-CSF administration.
    Blood. 118: 4209-14.
  11. Larsen, A.K. et al. (2013) Entry and Elimination of Marine Mammal Brucella spp. by Hooded Seal (Cystophora cristata) Alveolar Macrophages In Vitro.
    PLoS One. 8: e70186.
  12. Moreira, M.L. et al. (2016) Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes.
    Vet Parasitol. 220: 33-45.
  13. Bauer, T.R. Jr et al. (2017) Feline leukocyte adhesion (CD18) deficiency caused by a deletion in the integrin β2 (ITGB2) gene.
    Vet Clin Pathol. 46 (3): 391-400.
  14. Martini, V. et al. (2016) Canine small clear cell/T-zone lymphoma: clinical presentation and outcome in a retrospective case series.
    Vet Comp Oncol. 14 Suppl 1: 117-26.
  15. Brodzki, P. et al. (2020) Selected leukocyte subpopulations in peripheral blood and uterine washings in cows before and after intrauterine administration of cefapirin and methisoprinol.
    Anim Sci J. 91 (1): e13306.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Flow Cytometry

Synonyms
Integrin Beta 2 Chain
RRID
AB_2020973

MCA1780A647

148650 163620

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