Dinitrophenol antibody | LO-DNP-30

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Rat anti Dinitrophenol

Product Type
Monoclonal Antibody
Clone
LO-DNP-30
Isotype
IgE
Product Code Applications Pack Size List Price Quantity
MCA649G
Datasheet
E
SDS
0.25 mg loader

Rat anti Dinitrophenol antibody, clone LO-DNP-30 recognizes the dinitrophenyl (DNP) hapten.

Product Details

Target Species
Chemical
Product Form
Purified IgE - liquid
Preparation
Purified IgE prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
Nippostrongylus brasiliensis-DNP.
Approx. Protein Concentrations
IgE concentration 1.0 mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of Dinitrophenol antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 2.5 ug/ml (as detection antibody)
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Application Based External Images

Functional Assays

Product Specific References

References for Dinitrophenol antibody

  1. Pivniouk, V.I. et al. (1999) SLP-76 deficiency impairs signaling via the high-affinity IgE receptor in mast cells.
    J Clin Invest. 103 (12): 1737-43.
  2. Minas, I.S. et al. (2012) Physiological and proteomic approaches to address the active role of ozone in kiwifruit post-harvest ripening.
    J Exp Bot. 63 (7): 2449-64.
  3. Gilchrist, M. et al. (2010) A key role for ATF3 in regulating mast cell survival and mediator release.
    Blood. 115: 4734-41.
  4. Debrand, E. et al. (2019) An intergenic non-coding RNA promoter required for histone modifications in the human β-globin chromatin domain.
    PLoS One. 14 (8): e0217532.
  5. Okragly, A.J. et al. (2018) Human mast cells release the migraine-inducing factor pituitary adenylate cyclase-activating polypeptide (PACAP).
    Cephalalgia. 38 (9): 1564-74.

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