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Dinitrophenol antibody | LO-DNP-30

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Rat anti Dinitrophenol

Product Type
Monoclonal Antibody
Clone
LO-DNP-30
Isotype
IgE
Specificity
Dinitrophenol

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Product Code Applications Pack Size List Price Your Price Qty
MCA649G
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E 0.25 mg loader
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Dinitrophenol antibody, (clone LO-DNP-30) is a monoclonal antibody that recognizes the dinitrophenyl (DNP) hapten. The target is a small molecule commonly referred to as DNP or 2,4-dinitrophenyl.

This antibody has been reported to work in the following application; ELISA.

Dinitrophenyl is a low–molecular weight hapten that has no intrinsic biological function and is not endogenously expressed in mammalian tissues. It is widely used in immunology and biochemistry as a model hapten because it becomes immunogenic only when covalently conjugated to carrier proteins, such as serum albumin or hemocyanin, enabling the study of hapten–carrier immune responses. Antibodies raised against DNP–carrier conjugates specifically recognize the DNP group covalently attached to amino residues on the carrier molecule. The dinitrophenyl group binds covalently to amino groups on carrier molecules and is specifically recognized by anti-DNP antibodies generated against these DNP-carrier conjugates.

Target Species
Chemical
Product Form
Purified IgE - liquid
Preparation
Purified IgE prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
Nippostrongylus brasiliensis-DNP.
Approx. Protein Concentrations
IgE concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 2.5 ug/ml (as detection antibody)
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

References for Dinitrophenol antibody

  1. Pivniouk, V.I. et al. (1999) SLP-76 deficiency impairs signaling via the high-affinity IgE receptor in mast cells.
    J Clin Invest. 103 (12): 1737-43.
  2. Gilchrist, M. et al. (2010) A key role for ATF3 in regulating mast cell survival and mediator release.
    Blood. 115: 4734-41.
  3. Minas, I.S. et al. (2012) Physiological and proteomic approaches to address the active role of ozone in kiwifruit post-harvest ripening.
    J Exp Bot. 63 (7): 2449-64.
  4. Okragly, A.J. et al. (2018) Human mast cells release the migraine-inducing factor pituitary adenylate cyclase-activating polypeptide (PACAP).
    Cephalalgia. 38 (9): 1564-74.
  5. Debrand, E. et al. (2019) An intergenic non-coding RNA promoter required for histone modifications in the human β-globin chromatin domain.
    PLoS One. 14 (8): e0217532.

Functional Assays

Synonyms
DNP
RRID
AB_567293

MCA649G

PDF 160517 PDF 169966 PDF 180227

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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