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Ovalbumin antibody | 2C6

Mouse anti Ovalbumin

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price

Mouse anti Ovalbumin antibody, clone 2C6 recognises ovalbumin (OVA). The antibody is suitable for use as a mouse IgE standard in ELISA assays (Hamadaet al. 2003).

Target Species
Product Form
Purified IgE - liquid
Purified IgE prepared from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Approx. Protein Concentrations
IgE concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the mouse myeloma, P3U1.
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/5000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat anti Mouse IgE Heavy Chain:HRP MCA419P E 0.5 mg loader
List Price Your Price
Description Rat anti Mouse IgE Heavy Chain:HRP

Source Reference

  1. Hamada, K. et al. (2003) Allergen-independent maternal transmission of asthma susceptibility.
    J Immunol. 170: 1683-9.

References for Ovalbumin antibody

  1. Suzaki, Y. et al. (2005) A potent antiangiogenic factor, endostatin prevents the development of asthma in a murine model.
    J Allergy Clin Immunol. 116 (6): 1220-7.
  2. Fairley, K.J. et al. (2007) Exposure to the immunosuppressant, perfluorooctanoic acid, enhances the murine IgE and airway hyperreactivity response to ovalbumin.
    Toxicol Sci. 97 (2): 375-83.
  3. Chida, Y. et al. (2007) Early-life psychological stress exacerbates adult mouse asthma via the hypothalamus-pituitary-adrenal axis.
    Am J Respir Crit Care Med. 175: 316-22.
  4. Suzaki, Y. et al. (2007) A small-molecule compound targeting CCR5 and CXCR3 prevents airway hyperresponsiveness and inflammation.
    Eur Respir J. 31: 783-9.
  5. Kambayashi, T. et al. (2008) Indirect involvement of allergen-captured mast cells in antigen presentation.
    Blood. 111:1489-96.
  6. Stevens, T. et al. (2008) Increased transcription of immune and metabolic pathways in naive and allergic mice exposed to diesel exhaust.
    Toxicol Sci. 102: 359-70.
  7. Ellertsen,L.K. et al. (2010) Maternal allergen immunisation to prevent sensitisation in offspring: Th2-polarising adjuvants are more efficient than a Th1-polarising adjuvant in mice.
    BMC Immunol. 11: 8-17
  8. Paliwal, S. et al. (2010) One-step acquisition of functional biomolecules from tissues.
    Proc Natl Acad Sci U S A. 107: 14627-32.
  9. View The Latest Product References
  10. Cheung DS et al. (2010) Development of atopy by severe paramyxoviral infection in a mouse model.
    Ann Allergy Asthma Immunol. 105 (6): 437-443.e1.
  11. Piro,.B. et al. (2011) Towards the detection of human papillomavirus infection by a reagentless electrochemical peptide biosensor
    Electrochimica Acta. 56 (28): 10688-10693.
  12. Hansen, J.S. et al. (2011) Determinants of experimental allergic responses: interactions between allergen dose, sex and age.
    Scand J Immunol. 73 (6): 554-67.
  13. Nygaard, U.C. et al. (2015) Early life exposure to bisphenol A investigated in mouse models of airway allergy, food allergy and oral tolerance.
    Food Chem Toxicol. 83: 17-25.
  14. Andreassen, M. et al. (2015) Cry1Ab protein from Bacillus thuringiensis and MON810 cry1Ab-transgenic maize exerts no adjuvant effect after airway exposure.
    Scand J Immunol. 81 (3): 192-200.
  15. Shershakova N et al. (2015) Allergen-Specific Immunotherapy with Monomeric Allergoid in a Mouse Model of Atopic Dermatitis.
    PLoS One. 10 (8): e0135070.
  16. Diesner, S.C. et al. (2016) A distinct microbiota composition is associated with protection from food allergy in an oral mouse immunization model.
    Clin Immunol. 173: 10-18.
  17. Mothes, B. et al. (2016) p110γ/δ Double-Deficiency Induces Eosinophilia and IgE Production but Protects from OVA-Induced Airway Inflammation.
    PLoS One. 11 (7): e0159310.
  18. Garbani, M. et al. (2017) Allergen-loaded strontium-doped hydroxyapatite spheres improve allergen-specific immunotherapy in mice.
    Allergy. 72 (4): 570-8.
  19. Shin, al. (2018) V-set and Ig domain-containing 4 (VSIG4)-expressing hepatic F4/80+ cells regulate oral antigen-specific responses in mouse.
    Eur J Immunol. 48 (4): 632-43.
  20. Ghonim, M.A. et al. (2018) Sulfated non-anticoagulant heparin blocks Th2-induced asthma by modulating the IL-4/signal transducer and activator of transcription 6/Janus kinase 1 pathway.
    J Transl Med. 16 (1): 243.
  21. Haselmayer, P. et al. (2019) Efficacy and Pharmacodynamic Modeling of the BTK Inhibitor Evobrutinib in Autoimmune Disease Models.
    J Immunol. 202 (10): 2888-906.
  22. Boehne, C. et al. (2021) Tim-3 is dispensable for allergic inflammation and respiratory tolerance in experimental asthma.
    PLoS One. 16 (4): e0249605.
  23. Wilson, N.G. et al. (2023) The gut microbiota of people with asthma influences lung inflammation in gnotobiotic mice.
    iScience. 26 (2): 105991.
  24. Gnipp, S. et al. (2018) Nitric oxide dependent signaling via cyclic GMP in dendritic cells regulates migration and T-cell polarization.
    Sci Rep. 8 (1): 10969.
  25. Tsai, C.T. et al. (2018) Isotype-specific agglutination-PCR (ISAP): A sensitive and multiplex method for measuring allergen-specific IgE.
    J Allergy Clin Immunol. 141 (5): 1901-4.e15.



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