Ovalbumin antibody | 2C6
Mouse anti Ovalbumin antibody, clone 2C6 (MCA2259) used to generate a standard curve for murine IgE in a sandwich ELISA detected using biotin conjugated ovalbumin and streptavidin.
Image caption:
Analysis of OVA-specific IgE. The levels of IgE in sera obtained before, during, and after ASIT were detected and quantified by ELISA. The results are presented as mean IgE concentration (mean±SE, n = 8 for each). AD without ASIT: OVA-sensitized untreated mice; ASIT with OVA: OVA-sensitized mice treated by ASIT with OVA; ASIT with sOVA: OVA-sensitized mice treated by ASIT with sOVA; placebo: PBS-sensitized mice. (*, p<0.05).
From: Shershakova N, Bashkatova E, Babakhin A, Andreev S, Nikonova A, Shilovsky I,et al. (2015)
Allergen-Specific Immunotherapy with Monomeric Allergoid in a Mouse Model of Atopic Dermatitis. PLoS ONE 10(8): e0135070.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Ovalbumin antibody, clone 2C6 (MCA2259) used in an ELISA assay for the measurement of ovalbumin specific IgG in an experimental mouse model of airway inflammation.
Image caption:
Basal and OVA-specific IgE levels are increased in p110γ/δ-/- mice. Total and OVA-specific IgE was determined in sera of PBS-treated and OVA-treated KO and corresponding WT mice using ELISA. (A) Total serum IgE. (B) OVA-specific serum IgE. Bars express means + SD; n = 4–8 pooled from three independent experiments. Data were analysed by One-way ANOVA followed by Bonferroni's comparison tests for selected pairs of columns; **P<0.01, *P<0.05.
From: Mothes B, Bucher K, Ammon-Treiber S, Schwab M, Piekorz RP, Hirsch E, et al. (2016)
p110γ/δ Double-Deficiency Induces Eosinophilia and IgE Production but Protects from OVA-Induced Airway Inflammation.
PLoS ONE 11(7): e0159310.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Ovalbumin antibody, clone 2C6 (MCA2259) used as a control to assess anti ovalbumin IgE levels in murine broncholaralveolar lavage fluid by ELISA.
Image caption:
BALF analysis and OVA-specific immunoglobulin E.
(A, B) Bronchoalveolar lavage fluid was obtained from each individual mouse (WT, Tim-3-/-) at section day to determine total cell count (A) and number of eosinophils (B). No significant differences were detected between WT and Tim-3-/- OVA and TOL mice, resulting in comparable allergic and tolerant phenotypes. (Data of three independent experiments, n = 12-15 animals per group.) (C) Significant and comparable decrease in IgE production for WT and Tim-3-/- TOL mice compared to OVA controls. (One representative out of three independent experiments, n = 5-6 animals for each group.) Mann-Whitney-U-test. * p ≤0.05; ** p ≤0.01. All data are presented as mean ± SEM.
From: Boehne C, Behrendt A-K, Meyer-Bahlburg A, Boettcher M, Drube S, Kamradt T, et al.. (2021)
Tim-3 is dispensable for allergic inflammation and respiratory tolerance in experimental asthma. PLoS ONE 16(4): e0249605.
doi: 10.1371/journal.pone.0249605
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Ovalbumin antibody, clone 2C6 recognises ovalbumin (OVA). The antibody is suitable for use as a mouse IgE standard in ELISA assays (Hamadaet al. 2003).
Product Details
- Target Species
- Chicken
- Product Form
- Purified IgE - liquid
- Preparation
- Purified IgE prepared from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Ovalbumin.
- Approx. Protein Concentrations
- IgE concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the mouse myeloma, P3U1.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Ovalbumin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/1000 | 1/5000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rat anti Mouse IgE Heavy Chain:HRP | MCA419P | E | 0.5 mg |
|
Product Specific References
Source Reference
-
Hamada, K. et al. (2003) Allergen-independent maternal transmission of asthma susceptibility.
J Immunol. 170: 1683-9.
References for Ovalbumin antibody
-
Stevens, T. et al. (2008) Increased transcription of immune and metabolic pathways in naive and allergic mice exposed to diesel exhaust.
Toxicol Sci. 102: 359-70. -
Fairley, K.J. et al. (2007) Exposure to the immunosuppressant, perfluorooctanoic acid, enhances the murine IgE and airway hyperreactivity response to ovalbumin.
Toxicol Sci. 97 (2): 375-83. -
Ellertsen,L.K. et al. (2010) Maternal allergen immunisation to prevent sensitisation in offspring: Th2-polarising adjuvants are more efficient than a Th1-polarising adjuvant in mice.
BMC Immunol. 11: 8-17 -
Kambayashi, T. et al. (2008) Indirect involvement of allergen-captured mast cells in antigen presentation.
Blood. 111:1489-96. -
Paliwal, S. et al. (2010) One-step acquisition of functional biomolecules from tissues.
Proc Natl Acad Sci U S A. 107: 14627-32. -
Chida, Y. et al. (2007) Early-life psychological stress exacerbates adult mouse asthma via the hypothalamus-pituitary-adrenal axis.
Am J Respir Crit Care Med. 175: 316-22. -
Suzaki, Y. et al. (2007) A small-molecule compound targeting CCR5 and CXCR3 prevents airway hyperresponsiveness and inflammation.
Eur Respir J. 31: 783-9. -
Suzaki, Y. et al. (2005) A potent antiangiogenic factor, endostatin prevents the development of asthma in a murine model.
J Allergy Clin Immunol. 116 (6): 1220-7. -
Hansen, J.S. et al. (2011) Determinants of experimental allergic responses: interactions between allergen dose, sex and age.
Scand J Immunol. 73 (6): 554-67. -
Nygaard, U.C. et al. (2015) Early life exposure to bisphenol A investigated in mouse models of airway allergy, food allergy and oral tolerance.
Food Chem Toxicol. 83: 17-25. -
Shershakova N et al. (2015) Allergen-Specific Immunotherapy with Monomeric Allergoid in a Mouse Model of Atopic Dermatitis.
PLoS One. 10 (8): e0135070. -
Piro B. et al. (2011) Towards the detection of human papillomavirus infection by a reagentless electrochemical peptide biosensor
-
Diesner, S.C. et al. (2016) A distinct microbiota composition is associated with protection from food allergy in an oral mouse immunization model.
Clin Immunol. pii: S1521-6616(16)30300-X. [Epub ahead of print] -
Cheung DS et al. (2010) Development of atopy by severe paramyxoviral infection in a mouse model.
Ann Allergy Asthma Immunol. 105 (6): 437-443.e1. -
Garbani, M. et al. (2017) Allergen-loaded strontium-doped hydroxyapatite spheres improve allergen-specific immunotherapy in mice.
Allergy. 72 (4): 570-8. -
Mothes, B. et al. (2016) p110γ/δ Double-Deficiency Induces Eosinophilia and IgE Production but Protects from OVA-Induced Airway Inflammation.
PLoS One. 11 (7): e0159310. -
Mitragotri, S. et al. (2015) Compositions for Solubilizing Cells and/or Tissue
Pat app Pub: US 2015/0275174 A1 -
Andreassen, M. et al. (2015) Cry1Ab protein from Bacillus thuringiensis and MON810 cry1Ab-transgenic maize exerts no adjuvant effect after airway exposure.
Scand J Immunol. 81 (3): 192-200. -
Shin, W.et al. (2018) V-set and Ig domain-containing 4 (VSIG4)-expressing hepatic F4/80+ cells regulate oral antigen-specific responses in mouse.
Eur J Immunol. 48 (4): 632-43. -
Boehne, C. et al. (2021) Tim-3 is dispensable for allergic inflammation and respiratory tolerance in experimental asthma.
PLoS One. 16 (4): e0249605. -
Haselmayer, P. et al. (2019) Efficacy and Pharmacodynamic Modeling of the BTK Inhibitor Evobrutinib in Autoimmune Disease Models.
J Immunol. 202 (10): 2888-906. -
Ghonim, M.A. et al. (2018) Sulfated non-anticoagulant heparin blocks Th2-induced asthma by modulating the IL-4/signal transducer and activator of transcription 6/Janus kinase 1 pathway.
J Transl Med. 16 (1): 243.
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