Ovalbumin antibody | 2C6

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Mouse anti Ovalbumin

Product Type
Monoclonal Antibody
Clone
2C6
Isotype
IgE
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2259 E 0.1 mg
Mouse anti Ovalbumin antibody, clone 2C6 recognises ovalbumin (OVA). The antibody is suitable for use as a mouse IgE standard in ELISA assays (Hamadaet al. 2003).

Product Details

Target Species
Chicken
Product Form
Purified IgE - liquid
Preparation
Purified IgE prepared from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Ovalbumin.
Approx. Protein Concentrations
IgE concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the mouse myeloma, P3U1.

Storage Information

Storage
Store at +4oC or at -20oC if preferred. Avoid repeated freezing and thawing as this may denature the antibody.

Storage in frost free freezers is not recommended.

This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Ovalbumin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/5000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rat anti Mouse IgE Heavy Chain:HRP MCA419P 0.5 mg E

Product Specific References

Source Reference

  1. Hamada, K. et al. (2003) Allergen-independent maternal transmission of asthma susceptibility.
    J Immunol. 170: 1683-9.

References for Ovalbumin antibody

  1. Stevens, T. et al. (2008) Increased transcription of immune and metabolic pathways in naive and allergic mice exposed to diesel exhaust.
    Toxicol Sci. 102: 359-70.
  2. Fairley, K.J. et al. (2007) Exposure to the immunosuppressant, perfluorooctanoic acid, enhances the murine IgE and airway hyperreactivity response to ovalbumin.
    Toxicol Sci. 97 (2): 375-83.
  3. Ellertsen,L.K. et al. (2010) Maternal allergen immunisation to prevent sensitisation in offspring: Th2-polarising adjuvants are more efficient than a Th1-polarising adjuvant in mice.
    BMC Immunol. 11: 8-17
  4. Kambayashi, T. et al. (2008) Indirect involvement of allergen-captured mast cells in antigen presentation.
    Blood. 111:1489-96.
  5. Paliwal, S. et al. (2010) One-step acquisition of functional biomolecules from tissues.
    Proc Natl Acad Sci U S A. 107: 14627-32.
  6. Chida, Y. et al. (2007) Early-life psychological stress exacerbates adult mouse asthma via the hypothalamus-pituitary-adrenal axis.
    Am J Respir Crit Care Med. 175: 316-22.
  7. Suzaki, Y. et al. (2007) A small-molecule compound targeting CCR5 and CXCR3 prevents airway hyperresponsiveness and inflammation.
    Eur Respir J. 31: 783-9.
  8. Suzaki, Y. et al. (2005) A potent antiangiogenic factor, endostatin prevents the development of asthma in a murine model.
    J Allergy Clin Immunol. 116 (6): 1220-7.
  9. Hansen, J.S. et al. (2011) Determinants of experimental allergic responses: interactions between allergen dose, sex and age.
    Scand J Immunol. 73 (6): 554-67.
  10. Nygaard, U.C. et al. (2015) Early life exposure to bisphenol A investigated in mouse models of airway allergy, food allergy and oral tolerance.
    Food Chem Toxicol. 83: 17-25.
  11. Shershakova N et al. (2015) Allergen-Specific Immunotherapy with Monomeric Allergoid in a Mouse Model of Atopic Dermatitis.
    PLoS One. 10 (8): e0135070.
  12. Piro B. et al. (2011) Towards the detection of human papillomavirus infection by a reagentless electrochemical peptide biosensor
    Electrochimica Acta. 56 (28): 10688-93.
  13. Diesner, S.C. et al. (2016) A distinct microbiota composition is associated with protection from food allergy in an oral mouse immunization model.
    Clin Immunol. pii: S1521-6616(16)30300-X. [Epub ahead of print]
  14. Cheung DS et al. (2010) Development of atopy by severe paramyxoviral infection in a mouse model.
    Ann Allergy Asthma Immunol. 105 (6): 437-443.e1.
  15. Garbani, M. et al. (2016) Allergen-loaded strontium-doped hydroxyapatite spheres improve allergen-specific immunotherapy in mice.
    Allergy. Sep 3. [Epub ahead of print]
  16. Mothes, B. et al. (2016) p110γ/δ Double-Deficiency Induces Eosinophilia and IgE Production but Protects from OVA-Induced Airway Inflammation.
    PLoS One. 11 (7): e0159310.
  17. Mitragotri, S. et al. (2015) Compositions for Solubilizing Cells and/or Tissue
    Pat app Pub: US 2015/0275174 A1
  18. Andreassen, M. et al. (2015) Cry1Ab protein from Bacillus thuringiensis and MON810 cry1Ab-transgenic maize exerts no adjuvant effect after airway exposure.
    Scand J Immunol. 81 (3): 192-200.