MHC Class II Monomorphic antibody | 21-1A6
Mouse anti Chicken MHC Class II Monomorphic:FITC
- Product Type
- Monoclonal Antibody
- Clone
- 21-1A6
- Isotype
- IgG1
- Specificity
- MHC Class II Monomorphic
Mouse anti Chicken MHC Class II (monomorphic) antibody, clone 21-1A6, recognizes a monomorphic determinant on the chicken B-L molecule, the chicken class II major histocompatibility complex (MHC). The level of B-L expression is reported to increase during the bursal phase of B cell differentiation (Veromaa et al. 1988). |
- Target Species
- Chicken
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Chicken bursa cells
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for MHC Class II Monomorphic antibody
-
Veromaa, T. et al. (1988) Expression of B-L and Bu-1 antigens in chickens bursectomized at 60 h of incubation.
Eur J Immunol. 18 (2): 225-30. -
Vainio, O. et al. (1988) Antigen-presenting cell-T cell interaction in the chicken is MHC class II antigen restricted.
J Immunol. 140 (9): 2864-8. -
Silva, A.B. et al. (2008) Functional analysis of neuropeptides in avian thymocyte development.
Dev Comp Immunol. 32 (4): 410-20. -
Wattrang, E. (2009) Phosphorothioate oligodeoxyribonucleotides induce in vitro proliferation of chicken B-cells.
Vet Immunol Immunopathol. 131 (3-4): 218-28. -
Petkov, D.I. et al. (2009) Identification and characterization of two distinct bursal B-cell subpopulations following infectious bursal disease virus infection of White Leghorn chickens.
Avian Dis. 53 (3): 347-55. -
Pavlova, S.P. et al. (2010) In vitro and in vivo characterization of glycoprotein C-deleted infectious laryngotracheitis virus.
J Gen Virol. 91 (Pt 4): 847-57. -
Kamble, N.M. et al. (2016) Interaction of a live attenuated Salmonella gallinarum vaccine candidate with chicken bone marrow-derived dendritic cells.
Avian Pathol. 45 (2): 235-43. -
Kamble, N.M. et al. (2016) Activation of chicken bone marrow-derived dendritic cells induced by a Salmonella Enteritidis ghost vaccine candidate.
Poult Sci. 95 (10): 2274-80.
View The Latest Product References
-
Eren, U. et al. (2016) The several elements of intestinal innate immune system at the beginning of the life of broiler chicks.
Microsc Res Tech. 79 (7): 604-14. -
Jarosz, Ł. et al. et al. (2016) Effects of feed supplementation with glycine chelate and iron sulfate on selected parameters of cell-mediated immune response in broiler chickens.
Res Vet Sci. 107: 68-74. -
Jarosz, Ł.S. et al. (2018) The effect of feed supplementation with a copper-glycine chelate and copper sulphate on selected humoral and cell-mediated immune parameters, plasma superoxide dismutase activity, ceruloplasmin and cytokine concentration in broiler chickens.
J Anim Physiol Anim Nutr (Berl). 102 (1): e326-e336. -
Shojadoost, B. et al. (2019) Interactions between lactobacilli and chicken macrophages induce antiviral responses against avian influenza virus.
Res Vet Sci. 125: 441-50. -
Garrido, D. et al. (2018) The role of type I interferons (IFNs) in the regulation of chicken macrophage inflammatory response to bacterial challenge.
Dev Comp Immunol. 86: 156-70. -
Yildiz, M. et al. (2019) Histological and immunohistochemical studies of the proximal caecum and caecal tonsils of quail (Coturnix coturnix japonica).
Anat Histol Embryol. 48 (5): 476-85. -
Eren, U. et al. (2022) TLR2 and TLR4 molecules and antigen-presenting cell compositions in cecal tonsils of broiler chicks (Gallus gallus domesticus.) in the first two weeks of the post-hatch period.
Anat Histol Embryol. 51 (1): 125-135. -
Saint-Martin, V. et al. (2024) The gut microbiota and its metabolite butyrate shape metabolism and antiviral immunity along the gut-lung axis in the chicken.
Commun Biol. 7 (1): 1185.
- RRID
- AB_324487
MCA2171F
1607If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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