CD25 antibody | AbD13504

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Human anti Chicken CD25:Alexa Fluor® 647

Human anti Chicken CD25:FITC

Human anti Chicken CD25

Product Type
Monoclonal Antibody
Clone
AbD13504
Isotype
HuCAL Fab bivalent
Product Code Applications Pack Size List Price Quantity
HCA173A647
Datasheet
F
SDS
100 Tests/1ml loader

HCA173F
Datasheet
F
SDS
0.1 mg loader

HCA173
Datasheet
F
SDS
0.1 mg loader

Human anti Chicken CD25 antibody, clone AbD13504 recognizes the chicken IL-2 receptor alpha chain (IL-2Rα) , identified as the chicken homologue of human CD25 and cited as ChCD25 in published literature.
Chicken IL-2Rα is a cell surface antigen expressed by activated T-lymphocytes, monocytes/macrophages and thrombocytes.
CD4+ve/CD25+ve and CD8+ve/CD25+ve cells are up-regulated following infection with avian influenza virus (H9N2) and may therefore have an important role in the immune response to avian influenza virus infection (Huang et al. 2012).
Chicken IL-2Rα is the low affinity receptor for Interleukin-2.

Product Details

Target Species
Chicken
Species Cross-Reactivity
Target SpeciesCross Reactivity
Duck
Goose
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. Conjugated to Alexa Fluor 647 (A647) - liquid.
Product Form
A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. Conjugated to fluorescein isothiocyanate (FITC) - liquid.
Product Form
A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Preparation
Metal chelate affinity chromatography
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinantly expressed his-tagged chicken CD25
Approx. Protein Concentrations
Antibody concentration 0.05 mg/ml
Approx. Protein Concentrations
Antibody concentration 0.1 mg/ml
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
Q90WJ4
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of CD25 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry Neat 1/10
Flow Cytometry Neat 1/100
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Histidine Tag:Alexa Fluor® 488 MCA1396A488 F 100 Tests/1ml loader
Mouse anti Histidine Tag:Alexa Fluor® 647 MCA1396A647 F 1 ml loader
Mouse anti Histidine Tag:DyLight®800 MCA1396D800GA WB 0.1 mg loader
Mouse anti Histidine Tag:FITC MCA1396F F IF 0.1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
HuCAL Fab-dHLX-MH Negative Control:Alexa Fluor® 647 HCA052A647 F 1 ml loader
HuCAL Fab-dHLX-MH Negative Control:FITC HCA052F F 0.1 mg loader
HuCAL Fab-dHLX-MH Negative Control HCA052 E 0.1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Chicken CD4:FITC MCA2164F F 0.1 mg loader
Mouse anti Chicken CD4:RPE MCA2164PE F 100 Tests loader
Mouse anti Chicken CD8 Alpha:FITC MCA2166F F 0.1 mg loader
Mouse anti Chicken CD8 Alpha:RPE MCA2166PE F 100 Tests/1ml loader
Mouse anti Chicken CD25 MCA5925GA E F WB 0.1 mg loader
Mouse anti Chicken CD4:RPE MCA2164PE F 100 Tests loader
Mouse anti Chicken CD8 Alpha:RPE MCA2166PE F 100 Tests/1ml loader
Mouse anti Chicken CD25 MCA5925GA E F WB 0.1 mg loader
Mouse anti Chicken CD25 MCA5925GA E F WB 0.1 mg loader

Product Specific References

References for CD25 antibody

  1. Braukmann, M. et al. (2015) Avian CD25(+) gamma/delta (γδ) T cells after Salmonella exposure.
    Vet Immunol Immunopathol. 168 (1-2): 14-8.
  2. Polasky C et al. (2016) Non-specific activation of CD8α-characterised γδ T cells in PBL cultures of different chicken lines.
    Vet Immunol Immunopathol. 179: 1-7.
  3. Lee, I.K. et al. (2018) Regulation of CD4+CD8-CD25+ and CD4+CD8+CD25+ T cells by gut microbiota in chicken.
    Sci Rep. 8 (1): 8627.

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