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Bu-1A antibody | L22

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Anti Chicken Bu-1A Antibody, clone L22 thumbnail image 1 Anti Chicken Bu-1A Antibody, clone L22 thumbnail image 2 Anti Chicken Bu-1A Antibody, clone L22 thumbnail image 3 Anti Chicken Bu-1A Antibody, clone L22 thumbnail image 4 Anti Chicken Bu-1A Antibody, clone L22 thumbnail image 5
Anti Chicken Bu-1A Antibody, clone L22 gallery image 1
Figure A. FITC conjugated Mouse anti Chicken CD4 (MCA2164F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Chicken CD4 (MCA2164F) and RPE conjugated Mouse anti Chicken Bu-1A (MCA2170PE). All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes in the presence of 10% chicken serum. Data acquired on the ZE5 Cell Analyzer.
Anti Chicken Bu-1A Antibody, clone L22 gallery image 2
Figure A. Alexa Fluor 700 conjugated Rat anti Human CD3 and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. Alexa Fluor 700 conjugated Rat anti Human CD3 and RPE conjugated Mouse anti Chicken Bu-1A (MCA2170PE). All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes in the presence of 10% chicken serum. Data acquired on the ZE5 Cell Analyzer.
Anti Chicken Bu-1A Antibody, clone L22 gallery image 3
Figure A. Alexa Fluor 700 conjugated Rat anti Human CD3 and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. Alexa Fluor 700 conjugated Rat anti Human CD3 and FITC conjugated Mouse anti Chicken Bu-1A (MCA2170F). All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes in the presence of 10% chicken serum. Data acquired on the ZE5 Cell Analyzer.
Anti Chicken Bu-1A Antibody, clone L22 gallery image 4
Published customer image:
Mouse anti Chicken Bu-1 antibody, clone L22 (MCA2170F) used for immunofluorescence studies.
Image caption:
Confocal analysis of MacRed chicken post-hatch mononuclear phagocyte populations. (A) Splenic mononuclear phagocytes (red) and Bu-1+ B-cells (green) from a 16-week-old MacRed chicken. Rings of transgene-expressing cells can clearly be seen surrounding the ellipsoid (asterisk). (B) Bursa of Fabricius from an 8-day-old MacRed chicken: Bu-1+ B cells (green) show arrangement of the B-cell follicles; mononuclear phagocytes (red) are present in the medulla (M) and interfollicular region (red arrow), but not in the cortex (C) of B-cell follicles in the bursa of Fabricius. (C) Caecal tonsil B-cell follicle from a 10-week-old MacRed chicken, showing location of mononuclear phagocytes (red) and Bu-1+ B-cells (green). Transgene-expressing cells concentrated in the medulla region (M) of the B-cell follicle are a dense network of FDC. (D) Microglial cells (red) in the cerebellum of an 8-day-old MacRed chicken showing colocalisation with CD45 staining (green). (E) Kupffer cells (red) showing colocalisation with CSF1R (green) from a 13-week-old MacRed chicken liver. (F) Lung mononuclear phagocytes (red) and Bu-1+ B-cells (green) in the interstitial tissue of the parabronchial wall from a 16-week-old MacRed chicken. The parabronchial lumen (pb) is indicated. (G) Epidermal mononuclear phagocyte cells (red) in epidermal sheet preparation from a 10-week-old MacRed chicken. (H) Breast muscle mononuclear phagocytes (red) from a 16-week-old MacRed chicken co-expressing MHCII (green). (I) Feather pulp mononuclear phagocytes from an 8-day-old MacRed chicken (red) co-stained with CD45 (green). Scale bars: 50 μm.

From: Balic A, Garcia-Morales C, Vervelde L, Gilhooley H, Sherman A, Garceau V, Gutowska MW, Burt DW, Kaiser P, Hume DA, Sang HM.
Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
Development. 2014 Aug;141(16):3255-65.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Chicken Bu-1A Antibody, clone L22 gallery image 5
Published customer image:
Mouse anti Chicken Bu-1 antibody, clone L22 (MCA2170F) used for immunofluorescence studies.
Image caption:
F distribution of lymphoid aggregates in the MacRed chicken gut. (A-I) External views of different regions of a 1-year-old MacRed chicken showing several scattered lymphoid aggregates in the jejunum (A-C), numerous scattered lymphoid aggregates in the ileum (D-F) and a high concentration of lymphoid aggregates in the ileum Peyer's Patch. Scale bars in A-I: 500 μm. (J-L) Immunofluorescence staining of Peyers patches showing organisation of CSF1R-mApple-expressing cells (red) in relation to: (J) Bu-1+ B-cells (green), (K) TCR αββ (Vβ1)+ T-cells (green) and (L) CVI-ChNL-74.2+ macrophages (green). Scale bars in J-L: 100 μm.

From: Balic A, Garcia-Morales C, Vervelde L, Gilhooley H, Sherman A, Garceau V, Gutowska MW, Burt DW, Kaiser P, Hume DA, Sang HM.
Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
Development. 2014 Aug;141(16):3255-65.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

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F IF

Mouse anti Chicken Bu-1A:FITC

Product Type
Monoclonal Antibody
Clone
L22
Isotype
IgG1
Specificity
Bu-1A

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Product Code Applications Pack Size List Price Your Price Qty
MCA2170F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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loader

Mouse anti Chicken Bu-1a, clone L22 recognises the 'a' allele of the polymorphic cell surface antigen Bu-1, also known as chB6. Bu-1a is a 70 kDa alloantigen expressed on B cells and a subset of macrophages. Bu-1 is expressed on B cell precursors at early stages of development (Houssaint et al. 1991).
Weak cross-reactivity with Bu-1b may be seen at higher antibody levels. This may be removed by appropriate titration. Mouse anti chicken BU-1a, clone L22 cross reacts with quail Bu-1 but does not appear reactive with either turkey or guinea fowl tissues (Igyarto et al. 2008)

Target Species
Chicken
Species Cross-Reactivity
Target SpeciesCross Reactivity
Quail
Turkey
Guinea Fowl
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Immunogen
Chicken Bursa cells.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0-Ag14 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/20 1/80
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Source Reference

  1. Pink, J.R. & Rijnbeek, A.M. (1983) Monoclonal antibodies against chicken lymphocyte surface antigens.
    Hybridoma. 2: 287-96.

References for Bu-1A antibody

  1. Veromaa, T. et al. (1988) Monoclonal antibodies against chicken Bu-1a and Bu-1b alloantigens.
    Hybridoma. 7: 41-8.
  2. Houssaint, E. et al. (1989) Bu-1 antigen expression as a marker for B cell precursors in chicken embryos.
    Eur J Immunol. 19 (2): 239-43.
  3. Houssaint, E. et al. (1991) Early separation of B and T lymphocyte precursors in chick embryo.
    J Exp Med. 174: 397-406.
  4. Tregaskes, C.A. et al. (1996) Chicken B-cell marker chB6 (Bu-1) is a highly glycosylated protein of unique structure.
    Immunogenetics. 44: 212-7.
  5. Igyártó, B.Z. et al. (2008) Identification of the avian B-cell-specific Bu-1 alloantigen by a novel monoclonal antibody.
    Poult Sci. 87: 351-5.
  6. Chaussé, A.M. et al. (2011) Expression of toll-like receptor 4 and downstream effectors in selected cecal cell subpopulations of chicks resistant or susceptible to salmonella carrier state.
    Infect Immun. 79: 3445-54.
  7. Chaussé, A.M. et al. (2014) Susceptibility to Salmonella carrier-state: a possible Th2 response in susceptible chicks.
    Vet Immunol Immunopathol. 159: 16-28.
  8. Balic, A. et al. (2014) Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
    Development. 141: 3255-65.
    9. View The Latest Product References
  9. Luna-Acosta, J.L. et al. (2015) Direct antiapoptotic effects of growth hormone are mediated by PI3K/Akt pathway in the chicken bursa of Fabricius.
    Gen Comp Endocrinol. 224: 148-59.
  10. Jarosz, Ł. et al. (2017) The effect of feed supplementation with zinc chelate and zinc sulphate on selected humoral and cell-mediated immune parameters and cytokine concentration in broiler chickens.
    Res Vet Sci. 112: 59-65.
  11. Smialek, M. et al. (2017) Immunological aspects of the efficiency of protectotype vaccination strategy against chicken infectious bronchitis.
    BMC Vet Res. 13 (1): 44.
  12. Jarosz, Ł.S et al. (2018) The effect of feed supplementation with a copper-glycine chelate and copper sulphate on selected humoral and cell-mediated immune parameters, plasma superoxide dismutase activity, ceruloplasmin and cytokine concentration in broiler chickens.
    J Anim Physiol Anim Nutr (Berl). 102 (1): e326-e36.
  13. Härtle, S. et al. (2024) Delineation of chicken immune markers in the era of omics and multicolor flow cytometry
    Frontiers in Veterinary Science. 23 May [Epub ahead of print].

Flow Cytometry

Synonyms
Bursal Antigen 1 A
RRID
AB_2234729
UniProt
Q90746

MCA2170F

148226

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