Bu-1A antibody | L22

Mouse anti Chicken Bu-1A:FITC

Product Type
Monoclonal Antibody
Clone
L22
Isotype
IgG1
Specificity
Bu-1A

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MCA2170F
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Mouse anti Chicken Bu-1a, clone L22 recognises the 'a' allele of the polymorphic cell surface antigen Bu-1, also known as chB6. Bu-1a is a 70 kDa alloantigen expressed on B cells and a subset of macrophages. Bu-1 is expressed on B cell precursors at early stages of development (Houssaint et al. 1991).
Weak cross-reactivity with Bu-1b may be seen at higher antibody levels. This may be removed by appropriate titration. Mouse anti chicken BU-1a, clone L22 cross reacts with quail Bu-1 but does not appear reactive with either turkey or guinea fowl tissues (Igyarto et al. 2008)

Target Species
Chicken
Species Cross-Reactivity
Target SpeciesCross Reactivity
Quail
Turkey
Guinea Fowl
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Immunogen
Chicken Bursa cells.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0-Ag14 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/20 1/80
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

References for Bu-1A antibody

  1. Pink, J.R. & Rijnbeek, A.M. (1983) Monoclonal antibodies against chicken lymphocyte surface antigens.
    Hybridoma. 2: 287-96.
  2. Chaussé, A.M. et al. (2011) Expression of toll-like receptor 4 and downstream effectors in selected cecal cell subpopulations of chicks resistant or susceptible to salmonella carrier state.
    Infect Immun. 79: 3445-54.
  3. Houssaint, E. et al. (1989) Bu-1 antigen expression as a marker for B cell precursors in chicken embryos.
    Eur J Immunol. 19 (2): 239-43.
  4. Tregaskes, C.A. et al. (1996) Chicken B-cell marker chB6 (Bu-1) is a highly glycosylated protein of unique structure.
    Immunogenetics. 44: 212-7.
  5. Houssaint, E. et al. (1991) Early separation of B and T lymphocyte precursors in chick embryo.
    J Exp Med. 174: 397-406.
  6. Igyártó, B.Z. et al. (2008) Identification of the avian B-cell-specific Bu-1 alloantigen by a novel monoclonal antibody.
    Poult Sci. 87: 351-5.
  7. Veromaa, T. et al. (1988) Monoclonal antibodies against chicken Bu-1a and Bu-1b alloantigens.
    Hybridoma. 7: 41-8.
  8. Chaussé, A.M. et al. (2014) Susceptibility to Salmonella carrier-state: a possible Th2 response in susceptible chicks.
    Vet Immunol Immunopathol. 159: 16-28.
  9. View The Latest Product References
  10. Balic, A. et al. (2014) Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
    Development. 141: 3255-65.
  11. Luna-Acosta, J.L. et al. (2015) Direct antiapoptotic effects of growth hormone are mediated by PI3K/AKT pathway in the chicken bursa of Fabricius.
    Gen Comp Endocrinol. pii: S0016-6480(15)00204-X.
  12. Jarosz, Ł. et al. (2017) The effect of feed supplementation with zinc chelate and zinc sulphate on selected humoral and cell-mediated immune parameters and cytokine concentration in broiler chickens.
    Res Vet Sci. 112: 59-65.
  13. Smialek, M. et al. (2017) Immunological aspects of the efficiency of protectotype vaccination strategy against chicken infectious bronchitis.
    BMC Vet Res. 13 (1): 44.
  14. Jarosz, Ł.S et al. (2018) The effect of feed supplementation with a copper-glycine chelate and copper sulphate on selected humoral and cell-mediated immune parameters, plasma superoxide dismutase activity, ceruloplasmin and cytokine concentration in broiler chickens.
    J Anim Physiol Anim Nutr (Berl). 102 (1): e326-e36.

Flow Cytometry

Synonyms
Bursal Antigen 1 A
RRID
AB_2234729
UniProt
Q90746
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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