CD134 antibody | 7D6
lane 1 - Feline CD134 expressing cells
lane 2 - Control cells
lane 3 - Recombinant feline CD134-Fc fusion protein.
Mouse anti Cat CD134:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- 7D6
- Isotype
- IgG1
- Specificity
- CD134
Antibody Quality and Performance Guarantee
| Mouse anti Cat CD134 antibody, clone 7D6 recognizes feline CD134, otherwise known as OX40, a 43 kDa type I membrane protein and member of the tumor necrosis factor receptor superfamily, expressed predominantly by CD4+ activated T cells, and a key regulator of T cell-dependent immune responses. CD134 has been identified as the receptor for the OX40 ligand, CD252, expressed by activated B cells, acting as a co-stimulatory signal for the stimulation and secretion of immunoglobulins. CD134 has also been identified as a binding receptor for Feline Immunodeficiency Virus (FIV), acting along with CXC chemokine receptor 4 (CXCR4) to facilitate the entry of the virus into CD4+ primary target cells. |
- Target Species
- Cat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Weak Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin - Immunogen
- CHO-derived feline CD134-Fc fusion protein.
- Approx. Protein Concentrations
- IgG concentration 0.05mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the NS0 mouse myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/5 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA1209A647 | F | 100 Tests/1ml |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control:Alexa Fluor® 647 | ||||||
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
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| Description | Mouse IgG1 Negative Control:Alexa Fluor® 647 | ||||||
Source Reference
-
Willett, B.J. et al. (2007) Probing the interaction between feline immunodeficiency virus and CD134 by using the novel monoclonal antibody 7D6 and the CD134 (Ox40) ligand.
J Virol. 81: 9665-79.
References for CD134 antibody
-
Willett, B.J. et al. (2009) Enforced covalent trimerisation of soluble feline CD134 (OX40)-ligand generates a functional antagonist of feline immunodeficiency virus.
Mol Immunol. 46: 1020-30. -
McDonnel, S.J. et al. (2012) Pharmacologic reactivation of latent feline immunodeficiency virus ex vivo in peripheral CD4+ T-lymphocytes.
Virus Res. 170 (1-2): 174-9.
Further Reading
-
Stüber, E. & Strober, W. (1996) The T cell-B cell interaction via OX40-OX40L is necessary for the T cell-dependent humoral immune response.
J Exp Med. 183 (3): 979-89. -
de Parseval, A. et al. (2004) Feline immunodeficiency virus targets activated CD4+ T cells by using CD134 as a binding receptor.
Proc Natl Acad Sci U S A. 101 (35): 13044-9.
- RRID
- AB_2207244
MCA2568A647T
150870
MCA2568A647
150869
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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