Peptidoglycan antibody | 3F6B3 (10H6)

Mouse anti Peptidoglycan

Product Type
Monoclonal Antibody
Clone
3F6B3 (10H6)
Isotype
IgG1
Specificity
Peptidoglycan

Product Code Applications Pack Size List Price Your Price Qty
7263-1006
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Mouse anti peptidoglycan antibody, clone 3F6B3 recognizes the 3D polymer complex structure of peptidoglycan (PG). In a competitive immunoassay format, several compounds were found to be ineffective as inhibitors; muramyldipeptide, N-acetylglucosamine, chitin and acid hydrolyzed chitin. The epitope appears to consist of discontinuous glycan and/or amino acid residues.

Target Species
Bacterial
Product Form
Ascitic Fluid - raw
Preservative Stabilisers
None present.
Immunogen
This antibody was raised against insoluble peptidoglycan obtained by TCA-heat and ethanol extraction of Streptococcus mutans BHT cells.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1
  1. 1Treatment with strong acid, for Gram positive bacteria, or with a detergent, such as SDS, for Gram-negative bacteria may be necessary to expose the epitope.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

References for Peptidoglycan antibody

  1. Miklossy, J. et al. (2004) Borrelia burgdorferi persists in the brain in chronic lyme neuroborreliosis and may be associated with Alzheimer disease.
    J. Alzheimer's Dis. 6: 639-49.
  2. Wu, L. et al. (2007) Bacterial peptidoglycan breaks down intestinal tolerance via mast cell activation: the role of TLR2 and NOD2.
    Immunol Cell Biol. 85: 538-45.
  3. Rennemeier, C. et al. (2007) Thrombospondin-1 promotes cellular adherence of gram-positive pathogens via recognition of peptidoglycan.
    FASEB J. 21 (12): 3118-32.
  4. Schweitzer, M.H. et al. (2016) Testing the Hypothesis of Biofilm as a Source for Soft Tissue and Cell-Like Structures Preserved in Dinosaur Bone.
    PLoS One. 11 (2): e0150238.
  5. Miklossy J et al. (2008) Persisting atypical and cystic forms of Borrelia burgdorferi and local inflammation in Lyme neuroborreliosis.
    J Neuroinflammation. 5: 40.
  6. Robertson, J. et al. (2016) Intestinal APCs of the endogenous nanomineral pathway fail to express PD-L1 in Crohn's disease.
    Sci Rep. 6: 26747.
  7. Miklossy, J. (2016) Bacterial Amyloid and DNA are Important Constituents of Senile Plaques: Further Evidence of the Spirochetal and Biofilm Nature of Senile Plaques.
    J Alzheimers Dis. 53 (4): 1459-73.
  8. Miklossy, J. et al. (2008) Type 2 Diabetes: Local Inflammation and Direct Effect of Bacterial Toxic Components
    The Open Pathology Journal. 2 (1): 86-95.
  9. View The Latest Product References
  10. Van Gerven, N. et al. (2014) Secretion and functional display of fusion proteins through the curli biogenesis pathway.
    Mol Microbiol. 91 (5): 1022-35.
  11. Moon, M.S. et al. (2019) Bacterial Translocation and Host Immune Activation in Chronic Hepatitis C Infection.
    Open Forum Infect Dis. 6 (7) [Epub ahead of print].
  12. Lindgren, J. et al. (2017) Biochemistry and adaptive colouration of an exceptionally preserved juvenile fossil sea turtle.
    Sci Rep. 7 (1): 13324.

RRID
AB_620617
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