Peptidoglycan antibody | 3F6B3 (10H6)
Mouse anti Peptidoglycan antibody, clone 3F6B3 recognizes the 3D polymer complex structure of peptidoglycan (PG). In a competitive immunoassay format, several compounds were found to be ineffective as inhibitors; muramyldipeptide, N-acetylglucosamine, chitin and acid hydrolyzed chitin. The epitope appears to consist of discontinuous glycan and/or amino acid residues.
- Target Species
- Product Form
- Ascitic Fluid - raw
- Preservative Stabilisers
- None present.
- This antibody was raised against insoluble peptidoglycan obtained by TCA-heat and ethanol extraction of Streptococcus mutans BHT cells.
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- For research purposes only
Applications of Peptidoglycan antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
|Immunohistology - Paraffin 1|
- 1Treatment with strong acid, for Gram positive bacteria, or with a detergent, such as SDS, for Gram-negative bacteria may be necessary to expose the epitope.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Product Specific References
References for Peptidoglycan antibody
Miklossy, J. et al (2004) Borrelia burgdorferi persists in the brain in chronic lyme neuroborreliosis and may be associated with Alzheimer disease.
J. Alzheimer's Dis. 6: 639-649.
Wu, L. et al. (2007) Bacterial peptidoglycan breaks down intestinal tolerance via mast cell activation: the role of TLR2 and NOD2.
Immunol Cell Biol. 85: 538-45.
Rennemeier, C. et al. (2007) Thrombospondin-1 promotes cellular adherence of gram-positive pathogens via recognition of peptidoglycan.
FASEB J. 21 (12): 3118-32.
Schweitzer, M.H. et al. (2016) Testing the Hypothesis of Biofilm as a Source for Soft Tissue and Cell-Like Structures Preserved in Dinosaur Bone.
PLoS One. 11 (2): e0150238.
Miklossy J et al. (2008) Persisting atypical and cystic forms of Borrelia burgdorferi and local inflammation in Lyme neuroborreliosis.
J Neuroinflammation. 5: 40.
Robertson, J. et al. (2016) Intestinal APCs of the endogenous nanomineral pathway fail to express PD-L1 in Crohn's disease.
Sci Rep. 6: 26747.
Miklossy, J. (2016) Bacterial Amyloid and DNA are Important Constituents of Senile Plaques: Further Evidence of the Spirochetal and Biofilm Nature of Senile Plaques.
J Alzheimers Dis. 53 (4): 1459-73.
Miklossy, J. et al. (2008) Type 2 Diabetes: Local Inflammation and Direct Effect of Bacterial Toxic Components
The Open Pathology Journal. 2 (1): 86-95.
Van Gerven, N. et al. (2014) Secretion and functional display of fusion proteins through the curli biogenesis pathway.
Mol Microbiol. 91 (5): 1022-35.