Cathepsin L
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IF ResetMagic Red™ Cathepsin L Kit
- Product Type
- Kits
- Specificity
- Cathepsin L
Antibody Quality and Performance Guarantee
| The Magic Red Cathepsin L kit uses a quick and easy method to analyze intracellular cathepsin L protease activity in whole living cells. |
- Reagents in the Kit
- Pack Size: 25 TestsMagic Red Substrate (MR-FR2), 25 Tests - lyophilized
Hoechst 33342, 1 mL
Acridine Orange, 0.5 mLPack Size: 100 TestsMagic Red Substrate (MR-FR2), 100 Tests - lyophilized
Hoechst 33342, 1 mL
Acridine Orange, 0.5 ml. - Test Principle
- Cathepsin Magic Red kits measure cathepsin L protease activity by detecting active cathepsins in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of cathepsins to produce a fluorescent product.
The Magic Red reagent contains a cathepsin L target sequence peptide (FR)2 linked to a red (Cresyl Violet) fluorescent probe.
The Cathepsin Magic Red kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits.
This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates. - Regulatory
- For research purposes only.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Acknowledgements
- Magic Red is a trademark of Immunochemistry Technologies, LLC.
Store the unopened kit and each unopened component at +4°C until the expiration date.
Once reconstituted with DMSO, use Magic Red reagent immediately, or store at -20°C for 6 months protected from light and thawed no more than twice during that time.
Once reconstituted with DMSO, use Magic Red reagent immediately, or store at -20°C for 6 months protected from light and thawed no more than twice during that time.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT941.pdf
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT942.pdf
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Magic Red™ Cathepsin L Kit | ICT942 | IF | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Magic Red™ Cathepsin L Kit | ||||||
View more products with CATHEPSIN specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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