Cathepsin K

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Magic Red™ Cathepsin K Kit

Product Type
Kits
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
ICT939 IF 25 Tests
The Magic Red Cathepsin K kit uses a quick and easy method to analyze intracellular Cathepsin K protease activity in whole living cells.

Product Details

Reagents in the Kit
Magic Red™ MR-(LR)2 Reagent - lyophilized
Hoechst 33342 Stain, 1 mL
Acridine orange (AO), 0.5 mL
Test Principle
Cathepsin Magic Red™ kits measure cathepsin K protease activity by detecting active cathepsins in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of cathepsins to produce a fluorescent product.

The Magic Red™ reagent contains a cathepsin K target sequence peptide (LR)2 linked to a red (Cresyl Violet) fluorescent probe.

The Cathepsin Magic Red™ kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits.

This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates.

Storage Information

Storage
Store the unopened kit (and each unopened component) at +4oC until the expiration date.
Protect the MR-(LR)2 reagent from light at all times.

Once reconstituted, the 260X MR-(LR)2 stock should be stored at -20°C protected from light.
Shelf Life
Please see label for expiry date.

More Information

Acknowledgements
Magic Red™ is a trademark of Immunochemistry Technologies, LLC.
Regulatory
For research purposes only

Applications of Cathepsin K

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT939.pdf

Product Specific References

References for Cathepsin K

  1. Azuma, K. et al. (2007) Two osteoclastic markers expressed in multinucleate osteoclasts of goldfish scales.
    Biochem Biophys Res Commun. 362 (3): 594-600.
  2. de Vrieze, E. et al. (2010) ALP, TRAcP and cathepsin K in elasmoid scales: a role in mineral metabolism?
    Journal of Applied Ichthyology. 26 (2): 210-3.