The Magic Red™ Cathepsin K kit uses a quick and easy method to analyze intracellular Cathepsin K protease activity in whole living cells.
- Reagents in the Kit
- Magic Red™ MR-(LR)2 Reagent - lyophilized
Hoechst 33342 Stain, 1 mL
Acridine orange (AO), 0.5 mL
- Test Principle
- Cathepsin Magic Red™ kits measure cathepsin K protease activity by detecting active cathepsins in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of cathepsins to produce a fluorescent product.
The Magic Red™ reagent contains a cathepsin K target sequence peptide (LR)2 linked to a red (Cresyl Violet) fluorescent probe.
The Cathepsin Magic Red™ kits are suitable for cells in suspension, adherent cells, thin frozen tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits.
This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates.
- Store the unopened kit (and each unopened component) at +4oC until the expiration date.
Protect the MR-(LR)2 reagent from light at all times.
Once reconstituted, the 260X MR-(LR)2 stock should be stored at -20°C protected from light.
- Shelf Life
- Please see label for expiry date.
- Magic Red™ is a trademark of Immunochemistry Technologies, LLC.
- For research purposes only
Applications of Cathepsin K
|Application Name||Verified||Min Dilution||Max Dilution|
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT939.pdf
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for Cathepsin K
Azuma, K. et al. (2007) Two osteoclastic markers expressed in multinucleate osteoclasts of goldfish scales.
Biochem Biophys Res Commun. 362 (3): 594-600.
de Vrieze, E. et al. (2010) ALP, TRAcP and cathepsin K in elasmoid scales: a role in mineral metabolism?
Journal of Applied Ichthyology. 26 (2): 210-3.