Cathepsin K
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The Magic Red Cathepsin K Kit uses a quick and easy method to analyze intracellular cathepsin K protease activity in whole living cells.
Product Details
- Reagents in the Kit
- Magic Red Substrate (MR-LR2), 25 Tests - lyophilized
Hoechst 33342, 1 mL
Acridine Orange, 0.5 mL - Test Principle
- Cathepsin Magic Red Kits measure cathepsin K protease activity by detecting active cathepsins in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of cathepsins to produce a fluorescent product.
The Magic Red reagent contains a cathepsin K target sequence peptide (LR)2 linked to a red (Cresyl Violet) fluorescent probe.
The Cathepsin Magic Red Kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits.
This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates.
Storage Information
- Storage
- Store the unopened kit and each unopened component at +4oC until the expiration date.
Once reconstituted with DMSO, use Magic Red reagent immediately, or store at -20oC for 6 months protected from light and thawed no more than twice during that time. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- Acknowledgements
- Magic Red™ is a trademark of Immunochemistry Technologies, LLC.
- Regulatory
- For research purposes only
Applications of Cathepsin K
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT939.pdf
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for Cathepsin K
-
Azuma, K. et al. (2007) Two osteoclastic markers expressed in multinucleate osteoclasts of goldfish scales.
Biochem Biophys Res Commun. 362 (3): 594-600. -
de Vrieze, E. et al. (2010) ALP, TRAcP and cathepsin K in elasmoid scales: a role in mineral metabolism?
Journal of Applied Ichthyology. 26 (2): 210-3.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
