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Caspase-8

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FAM FLICA™ Caspase-8 Kit thumbnail image 1 FAM FLICA™ Caspase-8 Kit thumbnail image 2
FAM FLICA™ Caspase-8 Kit gallery image 1
Active caspase staining to measure apoptosis by flow cytometry
Jurkat cells were treated as shown with staurosporine at 1μM to induce apoptosis. The cells were then stained with FAM FLICA Caspase-8 Kit (ICT099) and ReadiDrop propidium iodide (1351101). Apoptotic cells positive for caspases can be seen in the bottom right quadrant and dead cells positive for both caspase and PI in the top right quadrant. Healthy cells are negative for both stains. Data acquired on the ZE5 Cell Analyzer
FAM FLICA™ Caspase-8 Kit gallery image 2
Both cells are apoptotic (green) and dying (blue nuclei). As the left cell is much brighter green than the right cell, the left cell had more active caspases.

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FAM FLICA™ Caspase-8 Kit

Product Type
Kits
Specificity
Caspase-8

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Product Code Applications Pack Size List Price Your Price Qty
ICT910
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF 100 Tests
List Price Your Price
ICT099
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF 25 Tests
List Price Your Price

The FAM FLICA Caspase-8 Kit uses a target sequence (LETD) sandwiched between a green fluorescent label, carboxyfluorescein (FAM), and a fluoromethylketone (FMK) to make a quick and flexible method to analyze active caspases in apoptotic cells.

Reagents in the Kit
Pack Size: 100 Tests
4 vials of FAM-LETD-FMK FLICA Reagent - lyophilized
10x Apoptosis Wash Buffer, 60 mL
Fixative, 6 mL
Propidium Iodide, 1 mL
Hoechst 33342, 1 mL
Pack Size: 25 Tests
1 vial of FAM-LETD-FMK FLICA Reagent - lyophilized
10x Apoptosis Wash Buffer, 15 mL
Fixative, 6 mL
Propidium Iodide, 1 mL
Hoechst 33342, 1 mL.
Test Principle
Caspase FLICA Kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a green (Carboxyfluorescein, FAM) fluorescent probe.

The Caspase FLICA Kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits.

This kit can be used with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FAM 494 520
Regulatory
For research purposes only.
Guarantee
Guaranteed until date of expiry. Please see product label.
Acknowledgements
FLICA is a trademark of Immunochemistry Technologies, LLC.

Pack Size: 100 Tests
Store the unopened kit and each unopened component at +4oC until the expiration date.
Once reconstituted with DMSO, use FLICA reagent immediately, or store at -20oC for 6 months protected from light and thawed no more than twice during that time.
Pack Size: 25 Tests
Store the unopened kit and each unopened component at +4°C until the expiration date.
Once reconstituted with DMSO, use FLICA reagent immediately, or store at -20°C for 6 months protected from light and thawed no more than twice during that time.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT910.pdf
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT099.pdf

Description Product Code Applications Pack Size List Price Your Price Quantity
FAM FLICA™ Caspase-8 Kit ICT910 F IF 100 Tests
List Price Your Price
Description FAM FLICA™ Caspase-8 Kit

References for Caspase-8

  1. Brugnon, F. et al. (2010) Apoptosis and meiotic segregation in ejaculated sperm from Robertsonian translocation carrier patients.
    Hum Reprod. 25 (7): 1631-42.
  2. Rébé C et al. (2007) Caspase-8 prevents sustained activation of NF-kappaB in monocytes undergoing macrophagic differentiation.
    Blood. 109 (4): 1442-50.
  3. Tazzari, P.L. et al. (2008) Synergistic proapoptotic activity of recombinant TRAIL plus the Akt inhibitor Perifosine in acute myelogenous leukemia cells.
    Cancer Res. 68 (22): 9394-403.
  4. Peppa, D. et al. (2013) Up-regulation of a death receptor renders antiviral T cells susceptible to NK cell-mediated deletion.
    J Exp Med. 210 (1): 99-114.
  5. Vacher, P. et al. (2015) Localized Store-Operated Calcium Influx Represses CD95-Dependent Apoptotic Effects of Rituximab in Non-Hodgkin B Lymphomas.
    J Immunol. 195 (5): 2207-15.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"