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Magic Red™ Caspase-3/7 Kit

Product Type
Product Code Applications Pack Size List Price Quantity
25 Tests

100 Tests

The Magic Red Caspase 3 & 7 kit uses a quick and easy method to analyze intracellular active caspases in apoptotic cells.

Product Details

Reagents in the Kit
Magic Red™ MR-(DEVD)2 Reagent - lyophilized
Hoechst 33342 Stain, 1 mL
Acridine orange (AO), 0.5 mL
Test Principle
Caspase Magic Red™ kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of caspases to produce a fluorescent product. The Magic Red™ reagent contains a caspase inhibitor sequence (DEVD)2 linked to a red (Cresyl Violet) fluorescent probe.

The Caspase Magic Red™ kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits.

This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates.

Storage Information

Store the unopened kit (and each unopened component) at +4oC until the expiration date.
Protect the MR-(DEVD)2 reagent from light at all times.

Once reconstituted, the 155X MR-(DEVD)2 stock should be stored at -20°C protected from light.
Guaranteed until date of expiry. Please see product label.

More Information

Magic Red™ is a trademark of Immunochemistry Technologies, LLC.
For research purposes only

Applications of Caspase-3/7

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Instructions For Use
Instructions for use can be found at

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Magic Red™ Caspase-3/7 Kit ICT936 IF 100 Tests

Product Specific References

References for Caspase-3/7

  1. Wei, Z. et al. (2011) Notch activation enhances the microglia-mediated inflammatory response associated with focal cerebral ischemia.
    Stroke. 42 (9): 2589-94.
  2. Shibata, Y. et al. (2016) Controlled in-cell release of caspase from photodegradable nanoparticles using the PARCEL method
    Applied Spectroscopy Reviews. Mar 13 [Epub ahead of print]