Caspase-2
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IF ResetFAM FLICA™ Caspase-2 Kit
- Product Type
- Kits
- Specificity
- Caspase-2
Antibody Quality and Performance Guarantee
| The FAM FLICA Caspase-2 Kit uses a quick and easy method to analyze active caspases in apoptotic cells. |
- Reagents in the Kit
- 1 vial of FAM-VDVAD-FMK FLICA Reagent - lyophilized
10x Apoptosis Wash Buffer, 15 mL
Fixative, 6 mL
Propidium Iodide, 1 mL
Hoechst 33342, 1 mL. - Test Principle
- Caspase FLICA kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a green (Carboxyfluorescein, FAM) fluorescent probe.
The Caspase FLICA kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits.
This kit can be used with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates. - Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FAM 494 520 - Regulatory
- For research purposes only.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Acknowledgements
- FLICA is a trademark of Immunochemistry Technologies, LLC.
Store the unopened kit and each unopened component at +4°C until the expiration date.
Once reconstituted with DMSO, use FLICA reagent immediately, or store at -20°C for 6 months protected from light and thawed no more than twice during that time.
Once reconstituted with DMSO, use FLICA reagent immediately, or store at -20°C for 6 months protected from light and thawed no more than twice during that time.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
||
| Immunofluorescence | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT918.pdf
ICT918
162416
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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