Caspase-10

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FAM FLICA™ Caspase-10 Kit

Product Type
Kits
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
ICT923 F IF 100 Tests
The FAM FLICA™ Caspase 10 kit uses a quick and easy method to analyze active caspases in apoptotic cells.

Product Details

Reagents in the Kit
4 vials of FAM-AEVD-FMK FLICA Reagent - lyophilized
10X Wash Buffer, 60 mL
Fixative, 6 mL
Propidium Iodide, 1 mL
Hoechst Stain, 1 mL
Test Principle
Caspase FLICA kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a green (Carboxyfluorescein, FAM) fluorescent probe.

The Caspase FLICA Kits are suitable for cells in suspension, adherent cells, thin tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits.

This kit can be used with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.

Storage Information

Storage
Store the unopened kit (and each unopened component) at +4oC until the expiration date.
Protect the FLICA reagent from light at all times.

Once reconstituted, the 150X FLICA stock should be stored at -20°C protected from light.
Shelf Life
Please see label for expiry date.

More Information

Acknowledgements
FLICA is a trademark of Immunochemistry Technologies, LLC.
Regulatory
For research purposes only

Applications of Caspase-10

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT923.pdf

Application Based External Images

Flow Cytometry

Product Specific References

References for Caspase-10

  1. Cisterne, A. et al. (2014) Silencer of Death Domains Controls Cell Death through Tumour Necrosis Factor-Receptor 1 and Caspase-10 in Acute Lymphoblastic Leukemia.
    PLoS One. 9: e103383.