Caspase-1

FLICA™ 660 Caspase-1 Kit

Product Type
Kits
Specificity
Caspase-1

Product Code Applications Pack Size List Price Your Price Qty
ICT9122
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF 25 - 50 Tests
List Price Your Price
Search for Batch Specific Datasheets

The FLICA 660 caspase-1 kit uses a target sequence (YVAD) sandwiched between a far-red fluorescent 660 dye and a fluoromethylketone (FMK) to make a quick and flexible method to analyze active caspase-1 in apoptotic cells.

Reagents in the Kit
1 vial of 660-YVAD-FMK FLICA Caspase-1 Reagent - lyophilized
10x Cellular Wash Buffer, 15 mL
Fixative, 6 mL
Test Principle
Caspase FLICA kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a far-red fluorescent 660 dye.

The Caspase FLICA Kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits. This kit can be used with either a flow cytometer or fluorescence microscope.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FLICA 660 660 685
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.
Acknowledgements
FLICA is a trademark of Immunochemistry Technologies, LLC.

Store the unopened kit and each unopened component at +4oC until the expiration date.
Once reconstituted with DMSO, use FLICA reagent immediately, or store at -20oC for 6 months protected from light and thawed no more than twice during that time.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT9122.pdf

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"