Caspase-1
FLICA™ 660 Caspase-1 Kit
- Product Type
- Kits
- Specificity
- Caspase-1
The FLICA 660 caspase-1 kit uses a target sequence (YVAD) sandwiched between a far-red fluorescent 660 dye and a fluoromethylketone (FMK) to make a quick and flexible method to analyze active caspase-1 in apoptotic cells. |
- Reagents In The Kit
- 1 vial of 660-YVAD-FMK FLICA Caspase-1 Reagent - lyophilized
10x Cellular Wash Buffer, 15 mL
Fixative, 6 mL - Test Principle
- Caspase FLICA kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a far-red fluorescent 660 dye.
The Caspase FLICA Kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits. This kit can be used with either a flow cytometer or fluorescence microscope. - Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FLICA 660 660 685 - Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Acknowledgements
- FLICA is a trademark of Immunochemistry Technologies, LLC.
Once reconstituted with DMSO, use FLICA reagent immediately, or store at -20oC for 6 months protected from light and thawed no more than twice during that time.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | |||
Immunofluorescence |
- Instructions For Use
-
ICT9122
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT9122.pdf
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
FLICA™ 660 Poly Caspase Kit | ICT9120 | F IF | 25 - 50 Tests | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | FLICA™ 660 Poly Caspase Kit | ||||||
FLICA™ 660 Caspase-3/7 Kit | ICT9125 | F IF | 25 - 50 Tests | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | FLICA™ 660 Caspase-3/7 Kit |
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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