The FAM FLICATM Caspase 1 kit uses a target sequence (YVAD) sandwiched between a green fluorescent label, carboxyfluorescein (FAM), and a fluoromethylketone (FMK) to make a quick and flexible method to analyze active caspases in apoptotic cells.
- Reagents in the Kit
- Pack Size: 25 Tests1 vial of FAM-YVAD-FMK FLICA™ Reagent - lyophilized
10X Wash Buffer, 15 mL
Fixative, 6 mL
Propidium Iodide, 1 mL
Hoechst Stain, 1 mLPack Size: 100 Tests4 vials of FAM-YVAD-FMK FLICA™ Reagent - lyophilized
10X Wash Buffer, 60 mL
Fixative, 6 mL
Propidium Iodide, 1 mL
Hoechst Stain, 1 mL
- Test Principle
- Caspase FLICA™ kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA™ reagent contains a caspase inhibitor sequence linked to a green (Carboxyfluorescein, FAM) fluorescent probe.
The Caspase FLICA™ Kits are suitable for cells in suspension, adherent cells, thin tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits.
This kit can be used with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.
- Store the unopened kit (and each unopened component) at +4oC until the expiration date.
Protect the FLICA™ reagent from light at all times.
Once reconstituted, the 150X FLICA™ stock should be stored at -20°C protected from light.
- Shelf Life
- Please see label for expiry date.
- FLICA™ is a trademark of Immunochemistry Technologies, LLC.
- For research purposes only
Applications of Caspase-1
|Application Name||Verified||Min Dilution||Max Dilution|
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT097.pdf
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|FAM FLICA™ Caspase-1 Kit||ICT098||100 Tests||F IF|
Product Specific References
References for Caspase-1
Hoegen, T. et al. (2011) The NLRP3 Inflammasome Contributes to Brain Injury in Pneumococcal Meningitis and Is Activated through ATP-Dependent Lysosomal Cathepsin B Release.
J Immunol. 187: 5440-51.
Edwards, M.R. et al. (2015) Metabolic dysfunction in lymphocytes promotes postoperative morbidity.
Clin Sci (Lond). Apr 20. [Epub ahead of print]
Inokuchi, T. et al. (2006) Plasma interleukin (IL)-18 (interferon-gamma-inducing factor) and other inflammatory cytokines in patients with gouty arthritis and monosodium urate monohydrate crystal-induced secretion of IL-18.
Cytokine. 33 (1): 21-7.
Hussen, J. et al. (2012) Inflammasome activation in bovine monocytes by extracellular ATP does not require the purinergic receptor P2X7.
Dev Comp Immunol. 38 (2): 312-20.
Wang, Y. et al. (2012) A comparative study of stress-mediated immunological functions with the adjuvanticity of alum.
J Biol Chem. 287 (21): 17152-60.
Wang, Y. et al. (2015) Stress activated DC induce dual homeostatic and inflammasome pathways, which may elicit CD4+ memory T cells and IFN stimulated genes.
J Biol Chem. pii: jbc.M115.645754.
Wree, A. et al. (2014) NLRP3 inflammasome activation results in hepatocyte pyroptosis, liver inflammation, and fibrosis in mice.
Hepatology. 59 (3): 898-910.
Sharma, A.A. et al. (2015) Impaired NLRP3 inflammasome activity during fetal development regulates IL-1β production in human monocytes.
Eur J Immunol. 45 (1): 238-49.
Gabrion, A. et al. (2016) mTOR inhibition counterbalances the inflammatory status of immune cells in Chronic Granulomatous Disease.
J Allergy Clin Immunol. pii: S0091-6749(16)31057-0. [Epub ahead of print]
Burm, S.M. et al. (2015) Inflammasome-induced IL-1β secretion in microglia is characterized by delayed kinetics and is only partially dependent on inflammatory caspases.
J Neurosci. 35 (2): 678-87.