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Caspase-1

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Both cells are apoptotic (green) and dying (blue nuclei). As the left cell is much brighter green than the right cell, the left cell had more active caspases.

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FAM FLICA™ Caspase-1 Kit

Product Type
Kits
Specificity
Caspase-1

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Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
ICT097
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF 25 Tests
List Price Your Price
ICT098
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF 100 Tests
List Price Your Price
Search for Batch Specific Datasheets

The FAM FLICA Caspase-1 Kit uses a target sequence (YVAD) sandwiched between a green fluorescent label, carboxyfluorescein (FAM), and a fluoromethylketone (FMK) to make a quick and flexible method to analyze active caspases in apoptotic cells.

Reagents in the Kit
Pack Size: 25 Tests
1 vial of FAM-YVAD-FMK FLICA Reagent - lyophilized
10x Apoptosis Wash Buffer, 15 mL
Fixative, 6 mL
Propidium Iodide, 1 mL
Hoechst 33342, 1 mL.
Pack Size: 100 Tests
4 vials of FAM-YVAD-FMK FLICA Reagent - lyophilized
10x Apoptosis Wash Buffer, 60 mL
Fixative, 6 mL
Propidium Iodide, 1 mL
Hoechst 33342, 1 mL.
Test Principle
Caspase FLICA kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a green (Carboxyfluorescein, FAM) fluorescent probe.

The Caspase FLICA Kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits.

This kit can be used with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FAM 494 520
Regulatory
For research purposes only.
Guarantee
Guaranteed until date of expiry. Please see product label.
Acknowledgements
FLICA is a trademark of Immunochemistry Technologies, LLC.

Store the unopened kit and each unopened component at +4°C until the expiration date.
Once reconstituted with DMSO, use FLICA reagent immediately, or store at -20°C for 6 months protected from light and thawed no more than twice during that time.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT097.pdf
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT098.pdf

Description Product Code Applications Pack Size List Price Your Price Quantity
FAM FLICA™ Caspase-1 Kit ICT098 F IF 100 Tests
List Price Your Price
Description FAM FLICA™ Caspase-1 Kit

References for Caspase-1

  1. Hoegen, T. et al. (2011) The NLRP3 Inflammasome Contributes to Brain Injury in Pneumococcal Meningitis and Is Activated through ATP-Dependent Lysosomal Cathepsin B Release.
    J Immunol. 187: 5440-51.
  2. Edwards, M.R. et al. (2015) Metabolic dysfunction in lymphocytes promotes postoperative morbidity.
    Clin Sci (Lond). Apr 20. [Epub ahead of print]
  3. Inokuchi, T. et al. (2006) Plasma interleukin (IL)-18 (interferon-gamma-inducing factor) and other inflammatory cytokines in patients with gouty arthritis and monosodium urate monohydrate crystal-induced secretion of IL-18.
    Cytokine. 33 (1): 21-7.
  4. Hussen, J. et al. (2012) Inflammasome activation in bovine monocytes by extracellular ATP does not require the purinergic receptor P2X7.
    Dev Comp Immunol. 38 (2): 312-20.
  5. Wang, Y. et al. (2012) A comparative study of stress-mediated immunological functions with the adjuvanticity of alum.
    J Biol Chem. 287 (21): 17152-60.
  6. Wang, Y. et al. (2015) Stress activated DC induce dual homeostatic and inflammasome pathways, which may elicit CD4+ memory T cells and IFN stimulated genes.
    J Biol Chem. pii: jbc.M115.645754.
  7. Wree, A. et al. (2014) NLRP3 inflammasome activation results in hepatocyte pyroptosis, liver inflammation, and fibrosis in mice.
    Hepatology. 59 (3): 898-910.
  8. Sharma, A.A. et al. (2015) Impaired NLRP3 inflammasome activity during fetal development regulates IL-1β production in human monocytes.
    Eur J Immunol. 45 (1): 238-49.
    9. View The Latest Product References
  9. Gabrion, A. et al. (2016) mTOR inhibition counterbalances the inflammatory status of immune cells in Chronic Granulomatous Disease.
    J Allergy Clin Immunol. pii: S0091-6749(16)31057-0. [Epub ahead of print]
  10. Burm, S.M. et al. (2015) Inflammasome-induced IL-1β secretion in microglia is characterized by delayed kinetics and is only partially dependent on inflammatory caspases.
    J Neurosci. 35 (2): 678-87.

Flow Cytometry

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"