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Caspase-1

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FAM FLICA™ Caspase-1 Kit gallery image 1
Both cells are apoptotic (green) and dying (blue nuclei). As the left cell is much brighter green than the right cell, the left cell had more active caspases

FAM FLICA™ Caspase-1 Kit

Product Type
Kits
Product Code Applications Pack Size List Price Quantity
ICT097
Datasheet
F IF 25 Tests

ICT098
Datasheet
F IF 100 Tests

The FAM FLICATM Caspase 1 kit uses a target sequence (YVAD) sandwiched between a green fluorescent label, carboxyfluorescein (FAM), and a fluoromethylketone (FMK) to make a quick and flexible method to analyze active caspases in apoptotic cells.

Product Details

Reagents in the Kit
Pack Size: 25 Tests
1 vial of FAM-YVAD-FMK FLICA™ Reagent - lyophilized
10X Wash Buffer, 15 mL
Fixative, 6 mL
Propidium Iodide, 1 mL
Hoechst Stain, 1 mL
Pack Size: 100 Tests
4 vials of FAM-YVAD-FMK FLICA™ Reagent - lyophilized
10X Wash Buffer, 60 mL
Fixative, 6 mL
Propidium Iodide, 1 mL
Hoechst Stain, 1 mL
Test Principle
Caspase FLICA™ kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA™ reagent contains a caspase inhibitor sequence linked to a green (Carboxyfluorescein, FAM) fluorescent probe.

The Caspase FLICA™ Kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits.

This kit can be used with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.

Storage Information

Storage
Pack Size: 25 Tests
Store the unopened kit (and each unopened component) at +4oC until the expiration date.
Protect the FLICA™ reagent from light at all times.

Once reconstituted, the 150X FLICA™ stock should be stored at -20°C protected from light.
Pack Size: 100 Tests
Store the unopened kit (and each unopened component) at +4°C until the expiration date.
Protect the FLICA™ reagent from light at all times.

Once reconstituted, the 150X FLICA™ stock should be stored at -20°C protected from light.
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

Acknowledgements
FLICA™ is a trademark of Immunochemistry Technologies, LLC.
Regulatory
For research purposes only

Applications of Caspase-1

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT097.pdf
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
FAM FLICA™ Caspase-1 Kit ICT098 F IF 100 Tests

Application Based External Images

Flow Cytometry

Product Specific References

References for Caspase-1

  1. Hoegen, T. et al. (2011) The NLRP3 Inflammasome Contributes to Brain Injury in Pneumococcal Meningitis and Is Activated through ATP-Dependent Lysosomal Cathepsin B Release.
    J Immunol. 187: 5440-51.
  2. Edwards, M.R. et al. (2015) Metabolic dysfunction in lymphocytes promotes postoperative morbidity.
    Clin Sci (Lond). Apr 20. [Epub ahead of print]
  3. Inokuchi, T. et al. (2006) Plasma interleukin (IL)-18 (interferon-gamma-inducing factor) and other inflammatory cytokines in patients with gouty arthritis and monosodium urate monohydrate crystal-induced secretion of IL-18.
    Cytokine. 33 (1): 21-7.
  4. Hussen, J. et al. (2012) Inflammasome activation in bovine monocytes by extracellular ATP does not require the purinergic receptor P2X7.
    Dev Comp Immunol. 38 (2): 312-20.
  5. Wang, Y. et al. (2012) A comparative study of stress-mediated immunological functions with the adjuvanticity of alum.
    J Biol Chem. 287 (21): 17152-60.
  6. Wang, Y. et al. (2015) Stress activated DC induce dual homeostatic and inflammasome pathways, which may elicit CD4+ memory T cells and IFN stimulated genes.
    J Biol Chem. pii: jbc.M115.645754.
  7. Wree, A. et al. (2014) NLRP3 inflammasome activation results in hepatocyte pyroptosis, liver inflammation, and fibrosis in mice.
    Hepatology. 59 (3): 898-910.
  8. Sharma, A.A. et al. (2015) Impaired NLRP3 inflammasome activity during fetal development regulates IL-1β production in human monocytes.
    Eur J Immunol. 45 (1): 238-49.
  9. Gabrion, A. et al. (2016) mTOR inhibition counterbalances the inflammatory status of immune cells in Chronic Granulomatous Disease.
    J Allergy Clin Immunol. pii: S0091-6749(16)31057-0. [Epub ahead of print]
  10. Burm, S.M. et al. (2015) Inflammasome-induced IL-1β secretion in microglia is characterized by delayed kinetics and is only partially dependent on inflammatory caspases.
    J Neurosci. 35 (2): 678-87.

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Protocols

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Downloads

Instructions For Use ICT097
Instructions For Use ICT098
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