TGF Alpha

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  • Recombinant Human TGF Alpha
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP037E, FN, WBdatasheet pdfdatasheet pdf0.1 mg
    PHP037
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Recombinant human TGFα is a 5.5kDa peptide corresponding to full-length mature human transforming growth factor alpha. TGFα is an EGF-related growth factor that stimulates the proliferation of epidermal and epithelial cells.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Recombinant protein - lyophilised
    • Reconstitution
      Reconstitute with 0.1ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage the addition of 0.1% bovine serum albumin (BSA) is recommended.
    • Preparation
      Recombinant protein expressed in E. coli.
    • Preservative Stabilisers
      None present
    • Activity
      >1.4 x 106 Units/mg
    • Purity
      >98% by SDS PAGE and HPLC
    • Approx. Protein Concentrations
      1.0 mg/ml after reconstitution
    • Protein Molecular Weight
      5.5kD (50 Amino Acid Sequence)
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <0.1ng/ug
    • Test Principle
    • Buffer Solution
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
    • GO Terms
      positive regulation of epidermal growth factor receptor activity
      MAP kinase kinase activity
      plasma membrane
      growth factor activity
      epidermal growth factor receptor binding
      positive regulation of epithelial cell proliferation
      positive regulation of mitosis
      positive regulation of cell division
      integral to membrane
      signal transducer activity
      activation of MAPK activity
      cell surface
      extracellular space
      cell proliferation
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4ng/well
      Functional Assays0.525ng/ml
      Western Blotting1.53.0ng/lane

    • Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      Recombinant human TGFα may be used as a standard for ELISA applications with either a purifed human TGFα antibody (AHP284G) or a biotinylated human TGFα antibody (AHP284B).
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Recombinant human TGFα may be used as the positive control for Western Blotting applications with either a purified human TGFα antibody (AHP284G) or a biotinylated human TGFα antibody (AHP284B).
    • Instructions For Use

    Additional TGF Alpha Formats

    Formats Applications Sizes available
    TGF Alpha : Rec. Protein E, FN, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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