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  • Rabbit anti Human TdT Kit
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  • Product Type
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    OBT0009IFdatasheet pdfdatasheet pdf datasheet pdf datasheet pdf20 Tests
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti Human TdT kit is a set of reagents intended to allow the identification of Terminal deoxynucleotidyl transferase positive (TdT+) cells by combined fluorescence and phase contrast microscopy.
    • Intended Use
    • Target Species
    • Product Form
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      All components contain 0.1% Sodium Azide (NaN3)
      Components 2, 2C and 3 contain 1% Bovine Serum Albumin
      Component M contains 80% glycerol
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
      Kit contains ready-to-use reagents sufficient for twenty slide tests:

      1 Normal goat serum - 1.0ml

      2 Rabbit anti- human -TdT antibody mixture - 0.5ml

      2C Negative control antibody - 0.5ml

      3 FITC-goat anti- rabbit IgG F(ab')2 - 1.0ml.

      M Mounting medium (80% glycerol in PBS/azide) - 1.0ml
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Additional Reagents Required
      Cold absolute methanol
      Phosphate buffered saline (PBS)
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      Please see label for expiry date.
    • GO Terms
      DNA binding
      DNA-directed DNA polymerase activity
      DNA modification
      DNA replication
      DNA nucleotidylexotransferase activity
      metal ion binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      Standard bone marrow or peripheral blood smears may be used for immunofluorescence staining. Cytocentrifuge preparations of cell suspensions made up in RPMI-1640 tissue culture medium containing 10% foetal calf serum are also suitable. Frozen sections should be cut as thin as possible. Store unfixed slide preparations in a dessicator at room temperature.

      1. Circle two areas of interest (1 cm diameter) with diamond pencil on the slide to be stained. One will be used as a control area and the other is for the test. Fix smears for 30 min. in absolute methanol at 4oC immediately before staining. Air dry.

      2. Block by adding a drop of normal goat serum to both circled areas. Let stand for 5 min. Rinse off goat serum with PBS.

      3. Apply 25 ul rabbit anti human TdT antibody mixture to the test area and 25 ul of negative control to control area and allow to react for 30 min in a humid chamber.

      4. Rinse avoiding cross-contamination of reagents. Now wash for 10 min. in a Coplin jar with several changes of PBS. Agitate occasionally for thorough washing.

      5. Wipe the slide, avoiding cells, and shake excess wash solution off circled area. Spread 25 ul of FITC goat anti rabbit IgG F(ab')2 and incubate in humid chamber for 30 min in the dark.

      6. Rinse off and wash for 10 min. in a Coplin jar with several changes of PBS.

      7. Apply small drops of glycerol/PBS (mounting medium) to two glass cover slips and invert circled areas on slide over dropped cover slips.

      8. Examine by epifluorescence and phase microscopy. Evaluate the percentage of nucleated cells exhibiting nuclear fluorescence.

    Additional TdT Formats

    Formats Applications Sizes available
    TdT : Kit IF 20 Tests
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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