Prostate Specific Antigen

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Published customer image:
Native Human prostate specific antigen(7820-0504) used for the evaluation of a biosensor performance for the identification and quantification of native PSA in human samples.
Image caption:
Initial test with PSA concentrations in PBS. (a) EIS spectra for: (i) buffer, (ii) 1?pg/ml, (iii) 10?pg/ml, (iv) 1?ng/ml and (v) 10?ng/ml; (b) normalized standard curve for data shown in the Figure 3(a).

From: Ganna Chornokur, Sunil K. Arya, Catherine Phelan, Richard Tanner, and Shekhar Bhansali,
“Impedance-Based Miniaturized Biosensor for Ultrasensitive and Fast Prostate-Specific Antigen Detection".
Journal of Sensors, vol. 2011, Article ID 983752.

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  • Native Human Prostate Specific Antigen
  • Native Human Prostate Specific Antigen
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  • Product Type
    Purified Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    7820-0504E, WBdatasheet pdfdatasheet pdf0.1 mg
    7820-0504
    7820-0604Edatasheet pdfdatasheet pdf0.2 mg
    7820-0604
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Native Human prostate specific antigen preparation is purigied form og human prostate-specific antigen (PSA), a glycoprotein of ~30 kDa found mainly in prostatic tissue and seminal fluid. PSA is also present in small quantities in the serum of normal men and is often elevated in the presence of prostate cancer and in other prostate disorders.

      Native Human prostate specific antigen demonstrates high specific activity and low levels of any contaminating protiens, inclding those with trypsin-like protease activity (Manning et al. 2012).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified protein from human seminal fluid - liquid
      Purified protein from human seminal fluid - liquid
    • Reconstitution
    • Preparation
      Pack Size: 0.2 mgSterile filtered through a 0.2 µM membrane
      Pack Size: 0.1 mgSterlie filtered through a 0.2 µM membrane
      Pack Size: 0.2 mgSterile filtered through a 0.2 µM membrane
      Pack Size: 0.1 mgSterlie filtered through a 0.2 µM membrane
    • Preservative Stabilisers
      Pack Size: 0.2 mg
      0.09%Sodium Azide (NaN3)
      Pack Size: 0.1 mg0.09% Sodium Azide (NaN3)
      Pack Size: 0.2 mg
      0.09%Sodium Azide (NaN3)
      Pack Size: 0.1 mg0.09% Sodium Azide (NaN3)
    • Purity
      Pack Size: 0.2 mg>40% by SDS-PAGE
      Pack Size: 0.1 mg>96% by SDS-PAGE
      Pack Size: 0.2 mg>40% by SDS-PAGE
      Pack Size: 0.1 mg>96% by SDS-PAGE
    • Approx. Protein Concentrations
      1.0 mg/ml
      1.0 mg/ml
    • Protein Molecular Weight
      30 kDa
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Pack Size: 0.2 mgStore at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.2 mgStore at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      12 months from date of despatch.
    • GO Terms
      extracellular region
      proteolysis
      negative regulation of angiogenesis
      serine-type endopeptidase activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Prostate Specific Antigen Formats

    Formats Applications Sizes available
    Prostate Specific Antigen : Purified E, WB 0.2 mg | 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Functional Assays
                Protein Purification

              References

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