Laminin

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  • Native Human Laminin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Purified Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    5620-0604E, FNdatasheet pdfdatasheet pdf0.1 mg
    5620-0604
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Native Human Laminin is purified protein from human placenta and supplied as a liquid.

      Laminin is an endothelial adhesion glycoprotein composed of 3 chains designated A (MW ~400 kDa), B1 (MW ~210 kDa) and B2 (MW ~200 kDa) held together by disulphide bonds in cruciform shape.

      Merck Index: 12: 5364
      CAS number: 114956-81-9
    • Intended Use
    • Target Species
      Human
    • Product Form
    • Reconstitution
    • Preparation
      Purified by immunoaffinity from pepsinized human placenta.
    • Preservative Stabilisers
      None present
    • Purity
      SDS PAGE: >90%
    • Approx. Protein Concentrations
      0.5 mg/ml
    • Molecular Weight
      In electrophoresis a major band is observed at 60 kDa and a minor band at 170-190 kDa.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Functional Assays

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      Care must be taken when using at room temperature. It is advisable to use the product as soon as possible. If a gel forms then it is not possible to reactivate for use.

    Additional Laminin Formats

    Formats Applications Sizes available
    Laminin : Purified E, FN 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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