IL-4

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  • Recombinant Human Interleukin-4
  • Recombinant Human Interleukin-4
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP234E, FN, WBdatasheet pdfdatasheet pdf20 µg
    PHP234
    PHP234AE, FN, WBdatasheet pdfdatasheet pdf5 µg
    PHP234A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Interleukin-4, also known as B-cell stimulatory factor 1, is a pleiotropic cytokine which interacts with multiple cell lineages, including T cells, fibroblasts and haematopoietic cells, and is one of the most important factors involved in B cell IgG1 and IgE class switching. Excessive secretion of IL-4 by Th2 helper cells, has been linked with allergy and elevated IgE levels.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified recombinant protein - lyophilised
      Purified recombinant protein - lyophilised
    • Reconstitution
      Pack Size: 20 µgReconstitute with 200ul distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      Pack Size: 5 µgReconstitute with 50ul distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

      For extended storage it is recommended to further dilute in a buffer containing 0.1% BSA.
      Pack Size: 20 µgReconstitute with 200ul distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      Pack Size: 5 µgReconstitute with 50ul distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

      For extended storage it is recommended to further dilute in a buffer containing 0.1% BSA.
    • Preparation
      Purified recombinant IL-4 expressed in E. coli.
      Purified recombinant IL-4 expressed in E. coli.
    • Preservative Stabilisers
      None present
      None present
    • Activity
      The ED50 as determined by the dose-dependent stimulation of human TF-1 cells is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 106 units/mg.
    • Purity
      >98% by SDS PAGE and HPLC
      >98% by SDS PAGE and HPLC
    • Approx. Protein Concentrations
      0.1mg/ml after reconstitution
      0.1mg/ml after reconstitution
    • Protein Molecular Weight
      14.9kD (129 amino acid residues)
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      < 1EU/ug
    • Test Principle
    • Buffer Solution
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution
      3 months from date of reconstitution
    • GO Terms
      transcription activator activity
      positive regulation of MHC class II biosynthetic process
      positive regulation of isotype switching to IgG isotypes
      negative regulation of osteoclast differentiation
      extracellular space
      positive regulation of transcription from RNA polymerase II promoter
      connective tissue growth factor biosynthetic process
      cytokine activity
      positive regulation of isotype switching to IgE isotypes
      negative regulation of apoptosis
      positive regulation of B cell proliferation
      growth factor activity
      type 2 immune response
      transcription repressor activity
      chemotaxis
      cholesterol metabolic process
      interleukin-4 receptor binding
      cellular defense response
      B cell differentiation
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4ng/well
      Functional Assays0.110.00ng/ml
      Western Blotting1.53.0ng/lane
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4ng/well
      Functional Assays0.110.00ng/ml
      Western Blotting1.53.0ng/lane

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      PHP234 may be used as a standard in ELISA application involving either AHP780 or AHP780B.
    • ELISA
      PHP234A may be used as a standard in ELISA application involving either AHP780 or AHP780B.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      PHP234 may be used as a positive control for western blotting applications involving either AHP780 or AHP780B.
    • Western Blotting
      PHP234A may be used as a positive control for western blotting applications involving either AHP780 or AHP780B.
    • Instructions For Use
    • Instructions For Use

    Additional IL-4 Formats

    Formats Applications Sizes available
    IL-4 : Rec. Protein E, FN, WB 20 µg | 5 µg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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