IL-1RA

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  • Recombinant Human Interleukin-1RA
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP080E, FN, WBdatasheet pdfdatasheet pdf20 µg
    PHP080
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Interleukin-1 receptor antagonist (IL-1RA) is a naturally occurring protein which inhibits the activity of IL-1 alpha and IL-1 beta by competitively blocking binding to cell surface receptors for IL-1.

      In an assay of dose dependent inhibition of IL-1 stimulation of D10S cells, the ED50 was expected to be 20-40ng/ml.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified recombinant protein - lyophilised
    • Reconstitution
      Reconstitute with 0.2ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% bovine serum albumin (BSA) is recommended.
    • Preparation
      Recombinant protein produced in E. coli
    • Preservative Stabilisers
      None present
    • Purity
      >98% by SDS PAGE
    • Approx. Protein Concentrations
      Total protein concentration 0.1mg/ml after reconstitution.
    • Protein Molecular Weight
      17kD/153 amino acids
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <0.1ng/ug
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution
    • GO Terms
      cytokine activity
      centrosome
      interleukin-1 receptor antagonist activity
      inflammatory response
      response to glucocorticoid stimulus
      plasma membrane
      immune response
      extracellular space
      nucleus
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4ng/well
      Functional Assays2040ng/ml
      Western Blotting1.53.0ng/lane

    • Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate positive/negative controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      Recombinant human Interleukin-1RA may be used as the standard for ELISA applications with either a purified human IL-1RA antibody or a biotinylated human IL-1RA antibody.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Recombinant huma Interleukin-1RA may be used as a positive control for Western Blotiing applications with either a purified human IL-1RA antibody or a biotinylated human IL-1RA antibody.
    • Instructions For Use

    Additional IL-1RA Formats

    Formats Applications Sizes available
    IL-1RA : Rec. Protein E, FN, WB 20 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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