PHP047A is a 161 amino acid recombinant IL-10 cytokine, with a range of reported properties including inhibition of macrophage mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cells response.
Recombinant human IL-10 has an ED50 of <2.0ng/ml as determined by co-stimulation of MC/9 cells with human IL-4.
Purified recombinant IL-10 - lyophilised
Reconstitute with 0.1ml of 5mM sodium phosphate pH 7.2. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
After the initial reconstitution step, the product should be diluted to a working concentration using an aqueous buffer containing 0.1% BSA (or any albumin), or diluted in culture media containing serum such as FCS.
Recombinant protein expressed in Escherichia coli
5 x 105 units/mg
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
Total protein concentration 0.1mg/ml after reconstitution.
Protein Molecular Weight
Reagents In The Kit
Preparing The Antibody
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate positive/negative controls.
PHP047A may be used in ELISA applications with either AHP795 or AHP795B.
Histology Positive Control Tissue
PHP047A may be used under reducing or non-reducing western blotting conditions with either AHP795 or AHP795B.