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  • Recombinant Human Interleukin-10
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP047AE, FN, WBdatasheet pdfdatasheet pdf10 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • PHP047A is a 161 amino acid recombinant IL-10 cytokine, with a range of reported properties including inhibition of macrophage mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cells response.

      Recombinant human IL-10 has an ED50 of <2.0ng/ml as determined by co-stimulation of MC/9 cells with human IL-4.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays0.220ng/ml
      Western Blotting1.53.0ng/lane

    • Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate positive/negative controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      PHP047A may be used in ELISA applications with either AHP795 or AHP795B.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      PHP047A may be used under reducing or non-reducing western blotting conditions with either AHP795 or AHP795B.
    • Instructions For Use

    Additional IL-10 Formats

    Formats Applications Sizes available
    IL-10 : Rec. Protein E, FN, WB 10 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rabbit anti Human Interleukin-10:BiotinAHP795B50 µgE, WB
          Rabbit anti Human Interleukin-10AHP7950.1 mgE, FN, WB

          Recommended Positive Controls

            Histology Controls

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