Human IgA1 | AbD264_hIgA

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Human IgA1 | AbD264_hIgA gallery image 1

ELISA analysis of recombinant Human IgA1 lambda

Recombinant Human IgA1 lambda detected with Mouse anti Human IgA1 alpha chain conjugated to biotin (913008) followed by HRP conjugated Streptavidin (STAR5B)

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SDS-PAGE analysis of recombinant Human IgA1 lambda

Recombinant Human IgA1 lambda was analyzed under non-reducing, denaturing conditions (Lane 2) and reducing, denaturing conditions (Lane 3). Bands are visualized using Coomassie staining and the heavy (HC) and light chains (LC) are indicated, respectively. The molecular weights (kDa) of the marker proteins (Lane 1) are indicated on the left hand side of the image

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Size exclusion chromatography of recombinant Human IgA1 lambda

UV280 chromatogram of a size exclusion chromatography run of recombinant Human IgA1 lambda. The larger peak corresponds to monomeric, fully assembled AbD264_hIgA. The smaller shoulder most likely contains dimers and aggregates

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  • Recombinant Human IgA1 Lambda
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
  • Clone
    AbD264_hIgA
  • Isotype
    IgA1 Lambda
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA172Edatasheet pdfdatasheet pdf0.1 mg
    HCA172
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Recombinant Human IgA1 lambda, clone AbD264_hIgA is a recombinant Human IgA1 antibody with a lambda light chain.

      Clone AbD264_hIgA is specific for green fluorescent protein (GFP). It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a control reagent for assays where this immunoglobulin is measured, or when using other human antibodies of the same isotype and subclass. It is recommended that this reagent is used at the same concentration as the test reagent.
    • Intended Use
    • Product Form
      Human IgA antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
    • Reconstitution
    • Preparation
      Purified IgA prepared by affinity chromatography.
    • Preservative Stabilisers
      0.01% Thiomersal
    • Immunogen
      Green Fluorescent Protein (GFP).
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 0.5 mg/ml.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
      antigen binding
      protein binding
      immune response
      protein-chromophore linkage
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Human IgA1 Formats

    Formats Clone Applications Sizes available
    Human IgA1 : Purified AbD264_hIgA E 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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