HSPA4 Antibody | AbD23625_hIgG1

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HSPA4 Antibody | AbD23625_hIgG1 gallery image 1

Western blot analysis was performed by loading 25µl MCF7 lysate (Lanes 2-7) onto an AnykD™ Criterion™ TGX Stain-Free™ gel. Precision Plus Protein™ Prestained Standards were run in lane 1. Lanes 2 & 3 and 5 & 6 were probed with Human anti HSPA4, clone AbD23625_hIgG1 (HCA273) at 1.25µg/ml (lanes 2 & 5) and 0.625µg/ml (lanes 3 & 6). Lanes 2-4 were probed with HRP conjugated Tidyblot™ (STAR209P) at 1/200. Lanes 5-7 were probed with HRP conjugated Goat anti Human IgG F(ab')2 antibody (STAR126P) at 1/2500. Visualization was carried out using the Bio-Rad Chemidoc™ Touch Imaging System

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  • Human anti HSPA4
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  • Product Type
    Monoclonal Antibody
  • Clone
    AbD23625_hIgG1
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA273WBdatasheet pdfdatasheet pdf0.1 mg
    HCA273
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Human anti HSPA4 antibody, clone AbD23625_hIgG1 recognizes human heat shock 70 kDa protein 4, also known as epididymis secretory sperm binding protein Li 5a and APG-2. It is an 840 amino acid chaperone that protects existing proteins against aggregation as well as mediating correct folding of newly synthesized proteins.

      HSPA4 has a predicted molecular weight of ~94 kDa; human anti HSPA4 antibody detects a band of approximately 110 kDa in western blotting.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      0.01% Thiomersal
    • Immunogen
      Recombinant human HSPA4
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      ATP binding
      protein import into mitochondrial outer membrane
      cellular chaperone-mediated protein complex assembly
      nucleus
      cytoplasm
      response to unfolded protein
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/501/800

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Anti HSPA4 antibody detects a band of approximately 110 kDa in MCF7 cell lysate under reducing conditions
    • Instructions For Use

    Additional HSPA4 Antibody Formats

    Formats Clone Applications Sizes available
    HSPA4 Antibody : Purified AbD23625_hIgG1 WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Goat anti Human IgG F(ab')2:HRPSTAR126P0.5 mlE, WB
          STAR126P
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

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