CTGF (connective tissue growth factor) is a heparin-binding pleiotropic growth factor and member of the CCN family of secreted cysteine rich regulatory proteins, which interacts with other growth factors including transforming growth factor beta (TGF-beta), vascular endothelial growth factor (VEGF) and bone morphogenetic proteins (BMPs).
CTGF promotes the adhesion of epithelial cells and fibroblasts, stimulates integrin expression, and promotes endothelial cell survival, migration and adhesion, implicating CTGF in angiogenesis. CTGF is involved in chondrogenesis and potentially skeletogenesis, induces extracellular matrix formation and has also been implicated in wound healing.
Purified recombinant protein - lyophilised
Reconstitute with 0.2ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Purified recombinant CTGF expressed in E.coli
Determined by the dose-dependent stimulation of the proliferation of HUVEC cells. The expected ED50 for this effect is 1.0-2.0 µg/ml.
>95% by SDS PAGE/HPLC analysis
Approx. Protein Concentrations
Total protein concentration 0.1mg/ml after reconstitution
11.2kD (98 amino acid residues)
Reagents In The Kit
Preparing The Antibody
10mM Sodium Acetate
Prior to reconstitution store at +4oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
PHP174 may be used as a standard in a sandwich ELISA with AHP1278 as a capture reagent and AHP1278B as the detection reagent.
Histology Positive Control Tissue
PHP174 may be used as a positive control for western blotting for either AHP1278 or AHP1278B.