Human collagen type IV from placenta.Human collagen type I-III, V, VI (<5%). Non collagen proteins (<0.5%).
Purified protein - liquid
Salt and alcohol precipitations, DEAE chromatography. Purity and retention of native helical structure was controlled by SDS-PAGE, ORD measurement, ability to form microfibrils and by reaction with anti-collagen type specific monoclonal antibodies.
0.09% Sodium Azide (NaN3)
SDS PAGE: >95%
Approx. Protein Concentrations
Total protein concentration 1.0mg/ml.
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
1. Takahashi, S. et al. (2015) C-type lectin-like domain and fibronectin-like type II domain of phospholipase A2 receptor 1 modulate binding and migratory responses to collagen.FEBS Lett. 589 (7): 829-35.
1. Glanville, R.W. et al. (1979) Isolation and characterization of a native placental basement-membrane collagen and its component alpha chainsEur. J. Biochem. 95: 383-389.
2. Sage, H. et al . (1979) Characterization of a novel collagen chain in human placenta and its relation to AB collagenBiochemistry. 18: 3815-3822.
3. Klasson, S.C. et al . (1986) The effects of tissue pretreatment and pepsin levels on the isolation of collagens from human placenta. Coll. Relat. Res. 6: 397-402.