CD274 Antibody

//www.bio-rad-antibodies.com/human-cd274-antibody-ahp1703.html?RenderPageSpecific=1
CD274 Antibody gallery image 1

Western blot analysis of whole cell lysate from Raji, human Burkitt's lymphoma derived cells probed with Rabbit anti Human CD274 (AHP1703) at 0.5(A) and 1(B) ug/ml

Enlarge
CD274 Antibody gallery image 2

Immunohistochemical staining of human heart with Rabbit anti Human CD274 (AHP1703)

Enlarge
CD274 Antibody gallery image 3

Published customer image:
Rabbit antiHuman CD274 antibody (AHP1703) used for the evaluation of PD-L1 expression on HCT-116 and HEK-293 cells by immunofluorescence and flow cytometry.
Image caption:
Immunofluorescent staining of HCT-116 and HEK-293 cells for PD-L1 (red), anti-CSV 84-1 (green), and nuclei (blue).
(A) Stains as a group of cells. (B) Stains of single cell. (C) HEK-293 cells stained for PD-L1 (red) and nuclei (blue). (D) HEK-293 cells transfected with PD-L1 stained for PD-L1 (red) and nuclei (blue). The images were taken using confocal microscopy. Scale bar, 10?μm. (E) Flow cytometric evaluation of intracellular PD-L1 expression vector control-transfected (left panel) and PD-L1–transfected (right panel) HEK-293 cells.

From: Satelli A, Batth IS, Brownlee Z, Rojas C, Meng QH, Kopetz S, Li S.
Potential role of nuclear PD-L1 expression in cell-surface vimentin positive circulating tumor cells as a prognostic marker in cancer patients.
Sci Rep. 2016 Jul 1;6:28910.

Enlarge
CD274 Antibody gallery image 4

Published customer image:
Rabbit antiHuman CD274 antibody (AHP1703) used for the evaluation of PD-L1 expression on circulating tumor cells from cancer patients by immunofluorescence.
Image caption:
PD-L1 expression in CTCs isolated from prostate cancer, breast cancer, colon cancer, and osteosarcoma patients.
Cells were stained for CSV (green) using the 84-1 antibody, PD-L1 (red), and SYTOX green (pseudo blue). The images were taken using confocal microscopy. Scale bar, 10?μm.

From: Satelli A, Batth IS, Brownlee Z, Rojas C, Meng QH, Kopetz S, Li S.
Potential role of nuclear PD-L1 expression in cell-surface vimentin positive circulating tumor cells as a prognostic marker in cancer patients.
Sci Rep. 2016 Jul 1;6:28910.

Enlarge
  • CD274 Antibody thumbnail image 1
  • CD274 Antibody thumbnail image 2
  • CD274 Antibody thumbnail image 3
  • CD274 Antibody thumbnail image 4
  • Rabbit anti Human CD274
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1703P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1703
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Human CD274 antibody detects CD274, also known as B7-H1 and PD-1L, a cell surface glycoprotein, a member of the B7 family of co-stimulatory molecules. CD274 is expressed constitutively on macrophages and dendritic cells, and is induced on activated T-cells, B-cells, endothelial cells and epithelial cells in response to Interferons alpha, beta and gamma. CD274 is reported to possess dual functions; inhibition of activated effector T cells and co-stimulation of naïve T cells. CD274 inhibits proliferation of activated T cells via ligation to the co-inhibitory molecule CD279 (programmed death-1; PD-1) leading to the secretion of the regulatory cytokine interleukin-10. CD274 has also been shown to co-stimulate early T cell priming and differentiation. Deregulated CD274 function has been reported in chronic viral and intracellular bacterial infection, as well as in many autoimmune diseases and cancers.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to Human CD274 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
    • Immunogen
      A peptide corresponding to a 17 amino acid sequence from near the centre of Human CD274.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      receptor activity
      integral to membrane
      T cell costimulation
      cell surface receptor linked signaling pathway
      endomembrane system
      cell proliferation
      immune response
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.51.0ug/ml
      Immunohistology - Paraffin(1)2.5ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human heart tissue
    • Immunofluorescence
    • Western Blotting
      AHP1703 detects a band of approximately 32kDa in Raji cell lysate.
    • Instructions For Use

    Additional CD274 Antibody Formats

    Formats Applications Sizes available
    CD274 Antibody : Purified P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Histar Detection SystemSTAR3000A50 TestsC, P
          STAR3000A
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Human heart tissue

              References

              Further Reading

              Write your review

              You may also be interested in...