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GST antibody | vpg66

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Mouse anti GST:Biotin

Product Type
Monoclonal Antibody
Clone
vpg66
Isotype
IgG1
Specificity
GST

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Product Code Applications Pack Size List Price Your Price Qty
MCA1352B
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Anti GST Antibody, clone vpg66 thumbnail image 1
Anti GST Antibody, clone vpg66 gallery image 1
Western blot analysis of GST ubiquitin recombinant protein probed with Mouse anti glutathione-S-transferase (MCA1352) followed by F(ab')2 Rabbit anti mouse IgG:HRP (STAR13B)

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WB

Mouse anti‑GST antibody (clone vpg66) is a monoclonal antibody that recognizes Glutathione S‑transferase (GST), also known as glutathione transferase. GST represents a family of cytosolic dimeric isoenzymes that catalyse the conjugation of reduced glutathione to a broad range of hydrophobic and electrophilic compounds.

GST is highly conserved across species, including mammals. and plays a central role in cellular detoxification and protection against chemical and oxidative stress. GSTs are widely expressed in tissues such as liver, kidney, intestines and lung, where they contribute to the metabolism and clearance of endogenous and exogenous compounds. Beyond their physiological role, GSTs are commonly used as fusion tags in recombinant protein expression systems.

Product Form
Purified IgG conjugated to biotin - liquid
Preparation
Antibody purified from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Immunogen
GST from Schistosoma japonicum.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 1/100 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

References for GST antibody

  1. Harris, M.P. & Neil, J.C. (1994) Myristoylation-dependent binding of HIV-1 Nef to CD4.
    J Mol Biol. 241 (2): 136-42.
  2. Heger, P. et al. (1998) Multimer formation is not essential for nuclear export of human T-cell leukemia virus type 1 Rex trans-activator protein.
    J Virol. 72: 8659-68.
  3. Elfgang, C. et al. (1999) Evidence for specific nucleocytoplasmic transport pathways used by leucine-rich nuclear export signals.
    Proc Natl Acad Sci U S A. 96: 6229-34.
  4. Lischka, P. et al. (2001) A novel transferable nuclear export signal mediates CRM1-independent nucleocytoplasmic shuttling of the human cytomegalovirus transactivator protein pUL69.
    EMBO J. 20: 7271-83.
  5. Davis, M.P. (2008) Recombinant VP4 of human rhinovirus induces permeability in model membranes.
    J Virol. 82: 4169-74.
  6. Shelton, H. & Harris, M. (2008) Hepatitis C virus NS5A protein binds the SH3 domain of the Fyn tyrosine kinase with high affinity: mutagenic analysis of residues within the SH3 domain that contribute to the interaction.
    Virol J. 5: 24.
  7. Foster, T.L. et al. (2011) Cyclophilin A interacts with domain II of hepatitis C virus NS5A and stimulates RNA binding in an isomerase-dependent manner.
    J Virol. 85: 7460-4.
  8. Blüggel, M. et al. (2011) Toward protein biomarkers for allergy: CD4+ T cell proteomics in allergic and nonallergic subjects sampled in and out of pollen season.
    J Proteome Res. 10 (4): 1558-70.

ELISA

Flow Cytometry

Immunofluorescence

Western Blotting

Synonyms
Glutathione-S-Transferase
RRID
AB_322967

MCA1352B

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"